ArticleSTAR protocols2026
Protocol for pH correction of FRET-based fluorescent biosensor imaging in dissociated Drosophila neurons.
Matthew S Price, Elham Rastegari, Kartik Venkatachalam
Abstract read
In one paragraphArticle in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 · Who and what moneyAuthors and funding
3 authors.
Matthew S PriceDepartment of Integrative Biology and Pharmacology, McGovern Medical School at the University of Texas Health Sciences Center (UTHealth), Houston, TX, USA; Neuroscience Graduate Program, The University of Texas MD Anderson Cancer Center UTHealth Houston Graduate School of Biomedical Sciences, Houston, TX, USA.
Elham RastegariDepartment of Integrative Biology and Pharmacology, McGovern Medical School at the University of Texas Health Sciences Center (UTHealth), Houston, TX, USA.
Kartik VenkatachalamDepartment of Integrative Biology and Pharmacology, McGovern Medical School at the University of Texas Health Sciences Center (UTHealth), Houston, TX, USA; Neuroscience Graduate Program, The University of Texas MD Anderson Cancer Center UTHealth Houston Graduate School of Biomedical Sciences, Houston, TX, USA; Molecular and Translational Biology Graduate Program, The University of Texas MD Anderson Cancer Center UTHealth Houston Graduate School of Biomedical Sciences, Houston, TX, USA. Electronic address: kartik.venkatachalam@uth.tmc.edu.
Funding
Neuropathology in tauopathies stem from depolarization-induced alterations in the planar distribution of phosphoinositidesRF1AG069076 · NIA · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI VENKATACHALAM, KARTIK · 2020 to 2020
$1.8MAlterations in somatodendritic bioenergetics in Drosophila models of tauopathyRF1AG072176 · NIA · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI VENKATACHALAM, KARTIK · 2021 to 2021
$1.2MAlterations in somatodendritic bioenergetics in Drosophila models of tauopathyR01AG072176 · NIA · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI KARTIK VENKATACHALAM · 2025 to 2026
$766kMetal responsive transcription factors in neurodegenerationR21AG087381 · NIA · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI VENKATACHALAM, KARTIK · 2025 to 2025
$429kNeuropathology in tauopathies stem from depolarization-induced alterations in the planar distribution of phosphoinositidesR01AG069076 · NIA · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI VENKATACHALAM, KARTIK · 2025 to 2025
$383kNIA NIH HHS R01 AG069076NIA NIH HHS R01 AG072176NIA NIH HHS R21 AG087381NIA NIH HHS RF1 AG069076NIA NIH HHS RF1 AG072176
6 · The paper itselfAbstract
Fluorescent biosensors are sensitive to intracellular pH fluctuations, which can shift fluorophore properties and compromise accurate metabolite quantification. We present a protocol for pH correction in FRET-based metabolic biosensor imaging using dissociated Drosophila larval neurons. We describe steps for integrating cytosolic pH measurements with R-based post hoc correction of FRET ratios. We also detail procedures for brain dissection, tissue dissociation, primary neuron culture, and live-cell imaging. This protocol enables accurate metabolic biosensor readouts at single-cell resolution. For complete details on the use and execution of this protocol, please refer to Price et al.
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Cell BiologyMicroscopyModel OrganismsNeuroscienceSingle Cell
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PMID42664056
PMCPMC13546833
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