Evidence map›Paper›PMID 42663964›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Droplet Contact Method (DCM) for Stable and Reproducible Lipid Bilayer Formation and its Application to Nanopore Measurements.

Nanami Takeuchi, Zugui Peng, Shoko Fujita, Mana Sato, Izumi Shibayama, Ryuji Kawano

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Nanami TakeuchiDepartment of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.
Zugui PengDepartment of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.
Shoko FujitaDepartment of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.
Mana SatoDepartment of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.
Izumi ShibayamaDepartment of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.
Ryuji KawanoDepartment of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan. rjkawano@cc.tuat.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Droplet contact bilayers provide a versatile platform for reconstituting and measuring the activity of membrane proteins at the single-molecule level. This method enables stable lipid bilayer formation between aqueous droplets and allows the insertion of protein nanopores for electrophysiological analysis. Here, we describe the fabrication of a poly(methyl methacrylate)-based microdevice with a parylene membrane, the formation of bilayers by the droplet contact method, and the reconstitution of both protein nanopores and de novo designed peptide nanopores. We further outline procedures for detecting peptide fragments and performing nanopore-based sequencing using this system. This approach offers reproducible bilayer assembly and quantitative analysis of ion channel activity, supporting the development of nanopore technologies for diverse molecular sensing and sequencing applications.

Indexed as

Lipid BilayersNanoporesLipid BilayersBilayer formationElectrophysiologyLipid bilayerLipid membraneMicrofluidicsNanopore sensingPlanar bilayerProtein nanopores

Identifiers

PMID42663964

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.