Evidence map›Paper›PMID 42663958›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Preparation and Detection of Designed DNA Carriers for Nanopore Sensing.

Yaxian Liu, Chengwei Luo, Ran Ji, Yufei Li, Yuxia Nie, Zemin Zhou, Rui Hu, Kaikai Chen

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yaxian LiuSchool of Nanoscience and Nanotechnology, University of Chinese Academy of Sciences, Beijing, 101408, China.
Chengwei LuoSchool of Nanoscience and Nanotechnology, University of Chinese Academy of Sciences, Beijing, 101408, China.
Ran JiSchool of Nanoscience and Nanotechnology, University of Chinese Academy of Sciences, Beijing, 101408, China.
Yufei LiSchool of Nanoscience and Nanotechnology, University of Chinese Academy of Sciences, Beijing, 101408, China.
Yuxia NieSchool of Nanoscience and Nanotechnology, University of Chinese Academy of Sciences, Beijing, 101408, China.
Zemin ZhouSchool of Nanoscience and Nanotechnology, University of Chinese Academy of Sciences, Beijing, 101408, China.
Rui HuSchool of Nanoscience and Nanotechnology, University of Chinese Academy of Sciences, Beijing, 101408, China. rui.hu@csu.edu.cn.
Kaikai ChenSchool of Nanoscience and Nanotechnology, University of Chinese Academy of Sciences, Beijing, 101408, China. chenkaikai@ucas.ac.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single-molecule detection is a powerful strategy for early disease diagnosis, allowing the specific identification of nucleic acids and proteins. While nanopore technology enables fluorescent label-free, high-resolution analysis of individual biomolecules, its lack of intrinsic molecular selectivity limits performance in complex biological samples. The DNA carrier strategy addresses this limitation by introducing target recognition capability, incorporating molecular barcodes, and enhancing signal clarity in complex backgrounds. When combined with low-noise, high-resolution glass nanopores, these carriers enable specific and multiplexed biomarker detection for early-stage cancer and other diseases. This chapter presents a detailed and standardized protocol for preparing and detecting assembled DNA carriers using nanopores, providing an accessible workflow for broader adoption in single-molecule sensing and molecular diagnostics.

Indexed as

Biosensing TechniquesDNANanoporesDNA NanostructuresHumansDNABiomarkerDNA carrier assemblyGlass nanoporeMolecular barcodingMolecular diagnosticsNanopore detectionSingle-molecule sensing

Identifiers

PMID42663958

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.