Evidence map›Paper›PMID 42663955›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Optical and Electrical Characterization of Nanopores in Freestanding Lipid Bilayers Formed on Microelectrode Cavity Arrays.

Madison A Handyside, Joseph S Harvey, SeeEun Lee, Kathryn A Michael, Kayla R Sennese, Annika E Evenson, Sarah E Koeneman, Daniel L Burden, Lisa M Keranen-Burden

Abstract read
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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Madison A Handyside *Wheaton College, Department of Chemistry, Wheaton, IL, 60187, USA.
Joseph S Harvey *Wheaton College, Department of Chemistry, Wheaton, IL, 60187, USA.
SeeEun Lee *Wheaton College, Department of Chemistry, Wheaton, IL, 60187, USA.
Kathryn A Michael *Wheaton College, Department of Chemistry, Wheaton, IL, 60187, USA.
Kayla R Sennese *Wheaton College, Department of Chemistry, Wheaton, IL, 60187, USA.
Annika E EvensonWheaton College, Department of Chemistry, Wheaton, IL, 60187, USA.
Sarah E KoenemanWheaton College, Department of Chemistry, Wheaton, IL, 60187, USA.
Daniel L BurdenWheaton College, Department of Chemistry, Wheaton, IL, 60187, USA. daniel.burden@wheaton.edu.
Lisa M Keranen-BurdenWheaton College, Department of Chemistry, Wheaton, IL, 60187, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single-channel electrical recording remains a fundamental technique for investigating biological nanopores embedded in lipid bilayer membranes. However, this method cannot reveal information from nanopores that fail to induce transmembrane ionic currents. Here, we present procedures that integrate a microelectrode cavity array with a single-molecule fluorescence microscope to probe labeled nanopores on freestanding lipid bilayers with light and electricity at the same time. Because oligomeric prepores are frequently employed in nanopore sensing applications, we explore the conversion of prepores into fully conducting pores. The protocol includes modified instructions for preparing and labeling alpha-hemolysin (αHL) heptamers, as well as a description of the instrument configuration and associated data analysis tools. We employ an Excel template (available as a supplemental file) to calculate a membrane-bound, but uninserted, heptameric nanopore diffusion coefficient of 8.1 ± 0.3 µm² s

Indexed as

Lipid BilayersNanoporesHemolysin ProteinsMicroelectrodesMicroscopy, FluorescenceSingle Molecule ImagingHemolysin ProteinsLipid BilayersAlpha hemolysinBilayerBiomembraneDiffusionIon-channel electrophysiologyMicroelectrode cavity arrayNanoporeSingle molecule fluorescenceSingle particle tracking

Identifiers

PMID42663955

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.