Evidence map›Paper›PMID 42663950›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Tuning Rectification and Gating in Biological Nanopores Through Lumen Charge Mutation.

Simon Finn Mayer, Marianna Fanouria Mitsioni, Maria Jose Marcaida, Theo Emmerich, Aleksandra Radenovic, Matteo Dal Peraro

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Simon Finn MayerInstitute of Bioengineering, School of Engineering, Swiss Federal Institute of Technology Lausanne, Lausanne, Switzerland.
Marianna Fanouria MitsioniInstitute of Bioengineering, School of Engineering, Swiss Federal Institute of Technology Lausanne, Lausanne, Switzerland.
Maria Jose MarcaidaInstitute of Bioengineering, School of Life Sciences, Swiss Federal Institute of Technology Lausanne, Lausanne, Switzerland.
Theo EmmerichLaboratoire de Physique, UMR CNRS 5672, ENS de Lyon, Université de Lyon, Lyon, France. theo.emmerich@epfl.ch.
Aleksandra RadenovicInstitute of Bioengineering, School of Engineering, Swiss Federal Institute of Technology Lausanne, Lausanne, Switzerland. aleksandra.radenovic@epfl.ch.
Matteo Dal PeraroInstitute of Bioengineering, School of Life Sciences, Swiss Federal Institute of Technology Lausanne, Lausanne, Switzerland. matteo.dalperaro@epfl.ch.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

β-barrel nanopores are involved in crucial biological processes, from ATP export in mitochondria to antibiotic resistance in Gram-negative bacteria, and represent a promising platform for emerging sequencing technologies. However, in contrast to ion channels, the understanding of the fundamental principles governing ion transport through these nanopores remains in its early stages. In this chapter, we describe the production and mutation of three major biological nanopores-aerolysin, MspA, and α-HL-as well as experimental approaches to elucidate ion transport mechanisms in these biological nanopores. Specifically, we provide tools to characterize two distinct nonlinear phenomena: open-pore rectification and gating, as well as guidelines to tune these phenomena to achieve a certain rectification or gating behavior.

Indexed as

Bacterial ToxinsEscherichia coli ProteinsHemolysin ProteinsIon Channel GatingMutationNanoporesPore Forming Cytotoxic ProteinsPorinsIon TransportBacterial ToxinsEscherichia coli ProteinsHemolysin ProteinsHlya protein, E colimspA protein, Mycobacterium smegmatisPore Forming Cytotoxic ProteinsPorinsstaphylococcal alpha-toxinBeta barrelBiological nanoporeGatingIon transportLumen chargeMutantRectification

Identifiers

PMID42663950

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.