Evidence map›Paper›PMID 42663949›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Expression and Purification of CytK Nanopores.

Nieck Jordy van der Heide, Andrea Bonini, Giovanni Maglia, Chunzhe Lu

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Nieck Jordy van der HeideChemical Biology, Groningen Biomolecular Sciences & Biotechnology Institute, University of Groningen, 9747 AG, Groningen, The Netherlands.
Andrea BoniniDepartment of Chemistry "Ugo Schiff", University of Florence, 50019, Sesto Fiorentino Florence, Italy.
Giovanni MagliaChemical Biology, Groningen Biomolecular Sciences & Biotechnology Institute, University of Groningen, 9747 AG, Groningen, The Netherlands.
Chunzhe LuChemical Biology, Groningen Biomolecular Sciences & Biotechnology Institute, University of Groningen, 9747 AG, Groningen, The Netherlands. chunzhe.lu@rug.nl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nanopores are emerging as powerful tools for peptide and protein sensing and sequencing. Cytotoxin K (CytK), a highly stable β-barrel pore, shows strong potential for both applications. The incorporation of an acidic-aromatic recognition site significantly enhances its ability to resolve different peptides. Other CytK variants with enhanced electroosmotic flow enable the measurement of unfolded native proteins. Together, these properties position CytK as an effective tunable nanopore for label-free detection of peptides and for analyzing native full-length proteins under denaturing conditions. This chapter provides a step-by-step protocol for the expression and purification of CytK nanopores.

Indexed as

NanoporesElectroosmosisGene ExpressionCytotoxin KElectroosmotic flowElectrophysiologyLabel-free detectionNanoporePeptide sequencingProtein identificationProtein purificationSingle-molecule

Identifiers

PMID42663949

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.