Evidence map›Paper›PMID 42663948›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Purification and Oligomerization of YaxAB Nanopores.

Marco van den Noort, Nieck Jordy van der Heide, Jakob H Viel, Sabine Straathof, Giovanni Maglia

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Marco van den NoortChemical Biology, Groningen Biomolecular Sciences & Biotechnology Institute, University of Groningen,, 9747 AG, Groningen, The Netherlands. marco.van.den.noort@rug.nl.
Nieck Jordy van der HeideChemical Biology, Groningen Biomolecular Sciences & Biotechnology Institute, University of Groningen,, 9747 AG, Groningen, The Netherlands.
Jakob H VielChemical Biology, Groningen Biomolecular Sciences & Biotechnology Institute, University of Groningen,, 9747 AG, Groningen, The Netherlands.
Sabine StraathofChemical Biology, Groningen Biomolecular Sciences & Biotechnology Institute, University of Groningen,, 9747 AG, Groningen, The Netherlands.
Giovanni MagliaChemical Biology, Groningen Biomolecular Sciences & Biotechnology Institute, University of Groningen,, 9747 AG, Groningen, The Netherlands.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Large nanopores are powerful tools for studying conformational dynamics of folded proteins on a single-molecule level. Additionally, they can be used as real-time metabolite sensors. YaxAB is a large funnel-shaped, hetero-oligomeric nanopore of variable size. It has a unique shape that enables the detection of a broad range of folded proteins and it can even provide insight into protein dimerization. Here, we present a straightforward protocol for the expression and purification of the monomeric components of YaxAB, as well as for their assembly into functional nanopores. Furthermore, we describe how specific oligomeric sizes of the nanopore can be enriched.

Indexed as

Escherichia coli ProteinsNanoporesProtein MultimerizationEscherichia coli ProteinsBiosensorElectrophysiologyNanoporeOligomerizationPore-forming toxinProtein purificationProtein trappingSingle-moleculeYaxAB

Identifiers

PMID42663948

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.