ArticleJournal of clinical microbiology2026
Seeing clearly without your lenses: digital holographic microscopy analysis of blood culture Gram stains.
Article in Journal of clinical microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Abstract
While automation and digital imaging technologies are now available for setup and workup of microbiology cultures, specimen Gram stain reading is still manually performed and requires experience to provide accurate results. In the article by K. P. Smith, E. Boulanger, M. Elkan, J. Fink, et al. (J Clin Microbiol 64:e01627-25, 2026, https://doi.org/10.1128/jcm.01627-25), the authors use a non-optical-based methodology, digital holographic microscopy, in combination with artificial intelligence to automate and provide interpretations and digital images of Gram stains from positive blood culture broths. While this differs from previous optical-based methods for Gram stain slide scanning, it demonstrated comparable performance. Gram stain slide staining and assistance by artificial intelligence for slide interpretation are a tantalizing future tool for clinical microbiology laboratories.
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Registered trials
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