Evidence map›Paper›PMID 42662905›Full record

ArticleVeterinary and animal science2026

Supplementation of lupeol during in vitro maturation improves preimplantation embryonic development in pigs.

Qingyi Lin, Takeshige Otoi, Oky Setyo Widodo, Theerawat Tharasanit, Kaywalee Chatdarong, Rentsenkhand Sambuu, Megumi Nagahara, Maki Hirata, Fuminori Tanihara, Zhao Namula

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Article in Veterinary and animal science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Qingyi LinDepartment of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, 524088, Guangdong, China.
Takeshige OtoiDepartment of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, 524088, Guangdong, China.
Oky Setyo WidodoFaculty of Veterinary Medicine, Universitas Airlangga, 60115, Surabaya, Indonesia.
Theerawat TharasanitFaculty of Veterinary Science, Chulalongkorn University, 10330, Bangkok, Thailand.
Kaywalee ChatdarongFaculty of Veterinary Science, Chulalongkorn University, 10330, Bangkok, Thailand.
Rentsenkhand SambuuInstitute for Extension of Advanced Agricultural Technology, 8098476, Ulaanbaatar, Mongolia.
Megumi NagaharaDepartment of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, 524088, Guangdong, China.
Maki HirataDepartment of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, 524088, Guangdong, China.
Fuminori TaniharaDepartment of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, 524088, Guangdong, China.
Zhao NamulaDepartment of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, 524088, Guangdong, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lupeol is a triterpene with several pharmacological properties, including antioxidant activity. This study investigated the antioxidant effects of lupeol supplementation during in vitro maturation (IVM) of porcine oocytes. Oocytes were divided into six groups and matured for 44 h in medium supplemented with lupeol at concentrations of 0, 1, 2, 4, and 8 µM. As a control, oocytes were cultured without lupeol or its dilution solution (dimethyl sulfoxide). After IVM, in vitro fertilization and embryo culture were performed to evaluate fertilization rate and developmental competence. Nuclear maturation, DNA integrity, intracellular glutathione (GSH) levels, reactive oxygen species (ROS) levels, fertilization, embryo development, and embryo quality were assessed. Lupeol at 4 and 8 µM increased metaphase II rates compared with the control group; however, the DNA integrity of the oocyte nucleus did not differ between groups. GSH levels increased, and ROS levels decreased at all concentrations compared with the control group. Monospermic fertilization rate of oocytes matured with 4 µM lupeol was higher than that of the 0 µM and control groups. Among the lupeol concentrations tested in IVM medium, only 4 µM significantly enhanced blastocyst formation. Moreover, 4 µM lupeol reduced DNA fragmentation in blastocysts compared with the 0 and 1 µM groups but did not affect the total cell number of blastocysts. In conclusion, the addition of 4 µM lupeol diluted in DMSO to the culture medium during IVM enhanced blastocyst formation by increasing intracellular GSH and reducing ROS in matured oocytes.

Indexed as

GSHLupeolPorcine embryoROSTriterpene

Identifiers

PMID42662905
PMCPMC13521140

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