Evidence map›Paper›PMID 42660881›Full record

ArticleNature communications2026

Weak SLP-76/PLC-γ1 interaction fine-tunes T cell receptor signal strength to optimize T cell responsiveness.

Hidehiro Yamane, Junya Wada, Elizabeth N Stassenko, Natalie D Convertino, Mariah E Lee, Melanie S Vacchio, Wenmei Li, Ferenc Livak, Udumbara M Rathnayake, Lakshmi Balagopalan and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors.

Hidehiro Yamane *Laboratory of Cellular and Molecular Biology, Center for Cancer Research (CCR), National Cancer Institute (NCI), National Institutes of Health (NIH), Bethesda, MD, USA. hidehiro.yamane@nih.gov.ORCID http://orcid.org/0009-0008-3282-2515
Junya Wada *Laboratory of Cellular and Molecular Biology, Center for Cancer Research (CCR), National Cancer Institute (NCI), National Institutes of Health (NIH), Bethesda, MD, USA.ORCID http://orcid.org/0009-0002-7281-5703
Elizabeth N StassenkoLaboratory of Cellular and Molecular Biology, Center for Cancer Research (CCR), National Cancer Institute (NCI), National Institutes of Health (NIH), Bethesda, MD, USA.
Natalie D ConvertinoLaboratory of Cellular and Molecular Biology, Center for Cancer Research (CCR), National Cancer Institute (NCI), National Institutes of Health (NIH), Bethesda, MD, USA.ORCID http://orcid.org/0009-0002-6034-7172
Mariah E LeeLaboratory of Cellular and Molecular Biology, Center for Cancer Research (CCR), National Cancer Institute (NCI), National Institutes of Health (NIH), Bethesda, MD, USA.ORCID http://orcid.org/0000-0002-2059-5801
Melanie S VacchioLaboratory of Integrative Cancer Immunology, CCR, NCI, NIH, Bethesda, MD, USA.
Wenmei LiLaboratory of Cellular and Molecular Biology, Center for Cancer Research (CCR), National Cancer Institute (NCI), National Institutes of Health (NIH), Bethesda, MD, USA.
Ferenc LivakFlow Cytometry Core, Laboratory of Genome Integrity, CCR, NCI, NIH, Bethesda, MD, USA.
Udumbara M RathnayakeLaboratory of Cellular and Molecular Biology, Center for Cancer Research (CCR), National Cancer Institute (NCI), National Institutes of Health (NIH), Bethesda, MD, USA.
Lakshmi BalagopalanLaboratory of Cellular and Molecular Biology, Center for Cancer Research (CCR), National Cancer Institute (NCI), National Institutes of Health (NIH), Bethesda, MD, USA.
Raj ChariGenome Modification Core, Laboratory of Animal Sciences Program (LASP), Frederick National Laboratory for Cancer Research (FNLCR), NCI, NIH, Frederick, MD, USA.ORCID http://orcid.org/0000-0002-2216-313X
Herbert HagenauMouse Modeling and Cryopreservation, LASP, FNLCR, NCI, NIH, Frederick, MD, USA.
Parirokh AwasthiMouse Modeling and Cryopreservation, LASP, FNLCR, NCI, NIH, Frederick, MD, USA.
Dorian B McGavernViral Immunology & Intravital Imaging Section, Neuroimmunology & Neurovirology Division, National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD, USA.ORCID http://orcid.org/0000-0001-9568-545X
Remy BosselutLaboratory of Integrative Cancer Immunology, CCR, NCI, NIH, Bethesda, MD, USA.
Lawrence E SamelsonLaboratory of Cellular and Molecular Biology, Center for Cancer Research (CCR), National Cancer Institute (NCI), National Institutes of Health (NIH), Bethesda, MD, USA. samelsonl@mail.nih.gov.ORCID http://orcid.org/0000-0002-5963-2112

Funding

Biochemical Basis of T Cell ActivationZIABC010304 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI SAMELSON, LAWRENCE · 2009 to 2025
$36.3M
Intramural NIH HHS ZIA BC010304
6 · The paper itself

Abstract

Upon T cell receptor (TCR) engagement, phosphorylation of the LAT adapter protein enables binding of the enzyme PLC-γ1 to a Gads/SLP-76 dimer, forming a tetrameric structure. The interaction between SLP-76 and PLC-γ1 is weak within this heterotetramer, and the relevant binding sites of SLP-76 and PLC-γ1 are highly conserved in vertebrates. We generated a mouse with a T cell-specific mutation in the SLP-76 that enhanced its affinity for PLC-γ1, thereby increasing PLC-γ1 activity and TCR signal strength. This mutation not only altered the development of αβTCR thymocytes, invariant NKT cells, and intraepithelial lymphocyte precursors but also impaired the generation of central memory CD8

Indexed as

Adaptor Proteins, Signal TransducingPhospholipase C gammaPhosphoproteinsReceptors, Antigen, T-CellT-LymphocytesAnimalsCD8-Positive T-LymphocytesMiceMice, Inbred C57BLMutationNatural Killer T-CellsProtein BindingSignal TransductionThymocytesAdaptor Proteins, Signal TransducingPhospholipase C gammaPhosphoproteinsReceptors, Antigen, T-CellSLP-76 signal Transducing adaptor proteins

Identifiers

PMID42660881
PMCPMC13522589

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.