Evidence map›Paper›PMID 42660116›Full record

ArticleStem cell reports2026

Bone marrow-inspired DLL1-presenting microparticles enhance asymmetric division and maintenance of mouse hematopoietic stem and progenitor cells.

Li Wenjing, Feng Yiting, Han Dongbo, Ao Yanxiao, Michael W Chen, Li Ning, Jin Yuhong, Liang Haiwei, Liu Wen, Zhu Xiaoyu and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Li WenjingSchool of Biomedical Engineering, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China.
Feng YitingSchool of Biomedical Engineering, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China.
Han DongboSchool of Biomedical Engineering, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China.
Ao YanxiaoSchool of Biomedical Engineering, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China.
Michael W ChenSchool of Biomedical Engineering, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China.
Li NingSchool of Biomedical Engineering, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China.
Jin YuhongSchool of Biomedical Engineering, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China.
Liang HaiweiSchool of Biomedical Engineering, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China.
Liu WenSchool of Biomedical Engineering, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China.
Zhu XiaoyuDepartment of Hematology, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China. Electronic address: xiaoyuz@ustc.edu.cn.
Du YananSchool of Biomedical Engineering, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China; National Key Laboratory of Kidney Diseases, Beijing, China. Electronic address: duyanan@tsinghua.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Understanding the regulation of hematopoietic stem and progenitor cell (HSPC) fate and translating it into effective culture strategies remains a significant challenge. Asymmetric lysosomal inheritance during HSPC division has been shown to predict variations in daughter cell activity and fate, yet the underlying regulators remain unclear. Through cell-cell communication analysis of bone marrow single-cell sequencing data, we identified the Notch ligand Delta-like protein 1 (DLL1) as a potential regulator in HSPC asymmetric division (ACD). Interactions between DLL1-presenting microparticles (MP-Ds) and HSPCs were observed in addressable microwell arrays, simulating cellular responses to localized niche signals. Long-term single-cell tracking revealed that MP-D interactions polarized HSPC lysosomes toward the contact site, directing division orientation and consequent asymmetric lysosomal inheritance in paired daughter cells. Furthermore, this co-culture system enhanced long-term hematopoietic reconstitution capacity of HSPCs in serial transplantations. Our findings support an association between HSPC ACD and the presentation mode of DLL1 signals, which enhances the ex vivo maintenance of HSPCs.

Indexed as

Asymmetric Cell DivisionBone MarrowCalcium-Binding ProteinsHematopoietic Stem CellsMembrane ProteinsAnimalsCell CommunicationLysosomesMiceStem Cell NicheCalcium-Binding ProteinsDlk1 protein, mouseMembrane Proteinsasymmetric divisionbone marrow nicheDelta-like protein 1DLL1hematopoietic stem cellsmicroparticles

Identifiers

PMID42660116
PMCPMC13555565

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.