Evidence map›Paper›PMID 42658688›Full record

ArticleSTAR protocols2026

Protocol for model-guided alternating high-temperature thermotherapy to eliminate major viruses from diverse sugarcane cultivars.

Guo-Qiang Huang, Jin-Long Guo, Feng Li, Qian Dong, Tung-Yu Hsieh

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Guo-Qiang HuangNational Engineering Research Center of Sugarcane, Fujian Agriculture and Forestry University, Fuzhou, China.
Jin-Long GuoNational Engineering Research Center of Sugarcane, Fujian Agriculture and Forestry University, Fuzhou, China.
Feng LiWuyou Ecological Agriculture Co., Ltd., Fuqing, China; Fujian Tianyuan Shiguang Leisure Agriculture Co., Ltd., Fuqing, China; Lanxi Agricultural Technology Co., Ltd., Fuqing, China; Cai Die Biological Technology Co., Ltd., Yongtai, China; Fujian Universities and Colleges Engineering Research Center of Modern Facility Agriculture, Fujian Polytechnic Normal University, Fuqing, China.
Qian DongSchool of Food and Biological Engineering, Fujian Polytechnic Normal University, Fuqing, China. Electronic address: dongqian@fpnu.edu.cn.
Tung-Yu HsiehWuyou Ecological Agriculture Co., Ltd., Fuqing, China; Fujian Tianyuan Shiguang Leisure Agriculture Co., Ltd., Fuqing, China; Lanxi Agricultural Technology Co., Ltd., Fuqing, China; Cai Die Biological Technology Co., Ltd., Yongtai, China; Fujian Universities and Colleges Engineering Research Center of Modern Facility Agriculture, Fujian Polytechnic Normal University, Fuqing, China; School of Food and Biological Engineering, Fujian Polytechnic Normal University, Fuqing, China; Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, China; Key Laboratory of Biological Breeding for Fujian and Taiwan Crops, Ministry of Agriculture and Rural Affairs, Fujian Agriculture and Forestry University, Fuzhou, China. Electronic address: dyxie@sibs.ac.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Vegetatively propagated sugarcane can accumulate multiple viruses that compromise planting-material health. Here, we present a model-guided alternating high-temperature thermotherapy (AHTT) protocol to eliminate major viruses from diverse sugarcane cultivars. We describe preparation and disinfection of single-bud axillary-bud cuttings, planting, thermal preconditioning, and a 12-day AHTT regimen. We then detail post-thermotherapy recovery, health scoring, leaf sampling, total-RNA extraction, control-validated one-step RT-PCR, result interpretation, and the detection of additional sugarcane viruses. For complete details on the use and execution of this protocol, please refer to Huang et al.

Indexed as

Biotechnology and bioengineeringMicrobiologyMolecular BiologyPlant sciences

Identifiers

PMID42658688
PMCPMC13546846

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.