Protocol for short-term in vitro propagation of patient-derived group 4 medulloblastoma MBT375 tumor cells.

Stefan Custers, Chitra Venugopal, Sheila K Singh

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Stefan CustersCentre for Discovery in Cancer Research, McMaster University, 1280 Main St W, Hamilton, ON L8S 4L8, Canada; Department of Surgery, McMaster University, 1280 Main St W, Hamilton, ON L8S 4L8, Canada. Electronic address: custerss@mcmaster.ca.

Chitra VenugopalCentre for Discovery in Cancer Research, McMaster University, 1280 Main St W, Hamilton, ON L8S 4L8, Canada; Department of Surgery, McMaster University, 1280 Main St W, Hamilton, ON L8S 4L8, Canada.

Sheila K SinghCentre for Discovery in Cancer Research, McMaster University, 1280 Main St W, Hamilton, ON L8S 4L8, Canada; Department of Surgery, McMaster University, 1280 Main St W, Hamilton, ON L8S 4L8, Canada; School of Cancer and Pharmaceutical Sciences, Comprehensive Cancer Centre, King's College London, London, UK. Electronic address: ssingh@mcmaster.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Durable in vitro cell culturing is an urgent unmet need for the pre-clinical development of novel group 4 medulloblastoma (G4-MB) specific therapies. Here, we present a protocol for the establishment and propagation of G4-MB tumor cells for short-term in vitro culturing. We describe brain tumor cell isolation, murine intracranial engraftment, in vitro culturing. and assay seeding. Our approach enables short-term in vitro passaging and drug screening. For long-term propagation of these G4-MB models, serial engraftment is required. For complete details on the use and execution of this protocol, please refer to Custers et al.

Indexed as

CancerCell BiologyCell cultureModel Organisms

Identifiers

PMID42658686

DOI10.1016/j.xpro.2026.104809

PMCPMC13546834

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.