ArticleTissue engineering and regenerative medicine2026
Peptide Dissolved in Alkaline Buffer with Blocking Significantly Enhances Performance of Synthetic Peptide-Displaying Surfaces in Supporting Human Pluripotent Stem Cell Culture.
Article in Tissue engineering and regenerative medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
backgroundHuman pluripotent stem cells (hPSCs) offer vast potential for cellular therapies due to their unique abilities in self-renewal and differentiation. However, the reliance on animal-derived Matrigel in conventional cell culture limits clinical translation, and developing chemically defined synthetic surfaces remains a key technical challenge.
methodsBased on our previously established synthetic polydopamine-based peptide-displaying surfaces, this study systematically optimized each fabrication step, including substrate selection, raw material screening (dopamine, carboxymethyl chitosan), reaction conditions, post-conjugation medium blocking, and peptide-dissolving buffer systems. Molecular docking was performed to analyze peptide-integrin αVβ5 interactions, and findings were verified using alginate-gelatin hydrogel and Maleylated-BSA coatings.
resultsCompared with commercial plates, pure polystyrene plates showed better abilities in sustaining cell culture, and the optimal raw materials for dopamine and carboxymethyl chitosan as well as their ideal reaction conditions were identified. Interestingly, not only does medium blocking after peptide conjugation play a crucial role, but the use of alkaline peptide-dissolving buffers also provides a significant advantage in supporting hPSCs adhesion and self-renewal. Molecular docking revealed enhanced peptide-integrin αVβ5 binding energetics under alkaline conditions. The optimized peptide-displaying surface demonstrated superior performance in supporting hPSCs culture and differentiation.
conclusionOur study reveals several previously unknown critical factors in the preparation of synthetic peptide-displaying surfaces. The optimized protocol improves hPSC culture performance and provides a reliable foundation for advancing chemically defined, animal component-free coatings toward commercial and clinical applications.
Indexed as
Identifiers
42658403What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.