ArticleVeterinary sciences2026
Stallion Sperm Lyophilisation: Effect of Source of Sperm and Reproductive Season.
Article in Veterinary sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
5 authors.
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Abstract
This study was conducted to study the quality and DNA integrity of freeze-dried stallion sperm, focusing on the influence of sperm origin (ejaculate vs. epididymis) and seasonality (breeding vs. non-breeding season). Ejaculates were collected from six healthy stallions and epididymal sperm were obtained from 12 stallions via cauda epididymis perfusion during the reproductive season and non-reproductive season in both cases. Sperm samples were diluted in an extender composed of 0.1 M trehalose and BSA 12.5% in PBS, freeze-dried, and stored at 4 °C for at least 3 months. After rehydration, sperm plasma membrane integrity and DNA integrity were analysed using eosin-nigrosin staining and a chromatin dispersion test (SCDt Halomax
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