Evidence map›Paper›PMID 42655697›Full record

ArticleViruses2026

Rapid Detection of N9 Subtype Avian Influenza Viruses Using an NA-Targeting Lateral Flow Immunoassay.

Qingmei Li, Yaning Sun, Yuhang Zhang, Zekun Meng, Yunrui Xing, Lu Fan, Jifei Yang, Junqing Guo

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Qingmei LiInstitute for Animal Health, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Yaning SunInstitute for Animal Health, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.ORCID 0000-0002-5512-4962
Yuhang ZhangInternational Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.
Zekun MengInternational Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.
Yunrui XingInstitute for Animal Health, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Lu FanInstitute for Animal Health, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Jifei YangInstitute for Animal Health, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Junqing GuoInstitute for Animal Health, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.ORCID 0000-0002-9862-3243

Funding

Henan Academy of Agricultural Sciences 2025JC16Henan Science and Technology Department 261111521400
6 · The paper itself

Abstract

The emergence of highly pathogenic avian influenza viruses (AIVs) poses significant threats to global public health and the poultry economy. Rapid detection methods targeting the hemagglutinin (HA) protein have become increasingly unreliable due to ongoing antigenic drift and genetic mutations. In this study, we screened two monoclonal antibodies (mAbs) against the highly conserved neuraminidase (NA) protein, which were characterized by high affinity and specificity for the N9 subtype. Using the paired mAbs 12A2 and 12F12, an NA-targeting lateral flow immunoassay (NA-LFIA) was developed for the rapid detection of N9 subtype AIVs. The NA-LFIA demonstrated exceptional specificity, exhibiting no cross-reactivity with the N1, N2, or N6 subtypes. The limits of detection (LODs) were established at 10

Indexed as

Influenza A virusInfluenza in BirdsNeuraminidaseViral ProteinsAnimalsAntibodies, MonoclonalAntibodies, ViralBirdsChickensImmunoassayRapid Diagnostic TestsSensitivity and SpecificityAntibodies, MonoclonalAntibodies, ViralNA protein, influenza A virusNeuraminidaseViral Proteinsavian influenza viruslateral flow immunoassaymonoclonal antibodiesN9 subtypeneuraminidase

Identifiers

PMID42655697
PMCPMC13517291

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.