ArticleMicroorganisms2026
Detection of Dengue Virus in
Article in Microorganisms, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Dengue remains one of the most important mosquito-borne viral diseases worldwide, and vector control continues to be the cornerstone of disease prevention. Vertical transmission has been proposed as a mechanism contributing to the maintenance of dengue virus (DENV) during interepidemic periods. In this study, field-collected egg pools and second-generation eggs derived from field-collected females from the state of Rio de Janeiro were analyzed by RT-qPCR, Sanger sequencing, plaque assay, and transmission electron microscopy. The second-generation egg samples (E1-E4), corresponding to F2 eggs of the AEDES-RIO strain, were all DENV positive. Sample E1 was positive for DENV-1, whereas samples E2-E4 were positive for DENV-4, confirming transovarial vertical transmission. The detection of DENV-1 and DENV-2 in field-collected egg pools further supports the occurrence of vertical transmission. All PCR-amplified viral fragments were confirmed by Sanger sequencing. Furthermore, homogenized egg samples naturally infected with DENV successfully infected Vero cells, demonstrating that the virus remained infectious. Transmission electron microscopy revealed virus-like particles of approximately 50 nm in diameter within egg tissues, providing ultrastructural evidence that corroborated the molecular and virological findings. Collectively, these findings provide additional evidence supporting vertical transmission of DENV in naturally infected
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Registered trials
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