Development of a Controlled-Dose Aerosol Challenge Method Using a Lung Fluid Quantitative Nebulizer.

Mingjie Shao, Huan Cui, Zhaoliang Chen, Lei Zhang, Cheng Zhang, Zhendong Guo, Peng Han

Abstract read

In one paragraph

Article in Microorganisms, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

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2 · The registry

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Mingjie ShaoCollege of Life Sciences, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China.

Huan CuiCollege of Veterinary Medicine, Hebei Agricultural University, 2596 Lucky South Street, Baoding 071000, China.

Zhaoliang ChenKey Laboratory of Jilin Province for Zoonoses Prevention and Control, State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, 573 Tulip Street, Changchun 130122, China.

Lei ZhangCollege of Veterinary Medicine, Hebei Agricultural University, 2596 Lucky South Street, Baoding 071000, China.

Cheng ZhangCollege of Veterinary Medicine, Hebei Agricultural University, 2596 Lucky South Street, Baoding 071000, China.

Zhendong GuoKey Laboratory of Jilin Province for Zoonoses Prevention and Control, State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, 573 Tulip Street, Changchun 130122, China.ORCID 0000-0001-8705-7744

Peng HanCollege of Life Sciences, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China.

Funding

National Natural Science Foundation of China 32573340

6 · The paper itself

Abstract

Airborne transmission represents a primary route of influenza virus dissemination. In animal infection models, conventional intranasal instillation fails to recapitulate the physiological features of natural respiratory infection. Existing aerosol-based challenge methods-including whole-body inhalation and oronasal exposure-face limitations in precise quantification of the delivered infectious dose. Moreover, oronasal exposure systems require specialized, high-cost instrumentation. This study established an economical and dosing-controlled aerosol challenge method utilizing a lung fluid quantitative nebulizer, which can deliver aerosolized virus directly to the lower respiratory tract via endotracheal intubation. The count median aerodynamic diameter (CMAD) of aerosols generated by the nebulizer was 2.28 μm. We compared pulmonary deposition efficiency and pathogenicity between lung-delivery aerosol inhalation and conventional intranasal instillation of PR8 influenza virus in mice. The pulmonary deposition rate for the aerosol inhalation was 63.1%, with an LD

Indexed as

influenza viruslung deliverypathogenicityquantitative nebulizerviral aerosols

Identifiers

PMID42655137

DOI10.3390/microorganisms14081793

PMCPMC13515033

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.