ArticleMicromachines2026
Photoinduced Fluorescence Changes in Selected Bacterial Strains Under 405 nm Laser Excitation: An In Vitro Hue-Based Monitoring Approach.
Article in Micromachines, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Bacterial fluorescence generated by endogenous fluorophores may provide an optical approach for monitoring microbial fluorescence responses. The aim of this study was to analyse temporal changes in the Hue parameter, defined as an image-derived component of the HSB colour space representing the dominant colour tone, and to characterize strain-associated fluorescence response profiles under 405 nm violet-light excitation. A total of 28 reference bacterial strains were examined. Each strain was inoculated at eight distinct locations on a separate BHI agar plate, yielding eight colonies per strain. At 24, 48, 72, 96, and 168 h of incubation, the same colonies were repeatedly exposed for 45 s to a 405 nm continuous-wave diode laser operating at 200 mW and imaged in a completely darkened room using an iPhone 14, a yellow emission filter, and fixed acquisition geometry. The JPEG images were converted to the HSB colour space in ImageJ software (version 1.54), and background-corrected Hue values were calculated for each colony as ΔHue = Hmean - Hmean_BG using the corresponding colony and agar-background regions of interest. A complementary qualitative visual assessment was performed, and aligned rank transform ANOVA was used to evaluate the effects of bacterial strain, incubation time, and their interaction. ΔHue values differed significantly between strains and across incubation time points, with significant strain × time interactions observed in both rod-shaped and coccal bacteria (all
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