Full-Length Transcriptome Reveals Alternative Splicing Regulation and Key Genes Underlying Differential Ginsenoside Accumulation Between Wild-Simulated and Cultivated Ginseng.

Xinyang Liu, Xinyi Zhao, Han Wang, Xiaoshuang Wei, Mengmei Hu, Yang Liu, Zhennan Wang, Dan Zhao, Zhenhui Wang

Abstract read

In one paragraph

Article in Biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Xinyang LiuFaculty of Agronomy, Jilin Agricultural University, Changchun 130118, China.

Xinyi ZhaoFaculty of Agronomy, Jilin Agricultural University, Changchun 130118, China.

Han WangFaculty of Agronomy, Jilin Agricultural University, Changchun 130118, China.

Xiaoshuang WeiFaculty of Agronomy, Jilin Agricultural University, Changchun 130118, China.

Mengmei HuFaculty of Agronomy, Jilin Agricultural University, Changchun 130118, China.

Yang LiuFaculty of Agronomy, Jilin Agricultural University, Changchun 130118, China.

Zhennan WangFaculty of Agronomy, Jilin Agricultural University, Changchun 130118, China.

Dan ZhaoGinseng and Antler Products Testing Center of the Ministry of Agricultural and Rural Affairs of the People's Republic of China, Jilin Agricultural University, Changchun 130118, China.

Zhenhui WangFaculty of Agronomy, Jilin Agricultural University, Changchun 130118, China.ORCID 0000-0002-1332-4480

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ginsenoside accumulation is tissue- and germplasm-specific, yet its transcriptional regulation remains unclear. We combined HPLC, PacBio full-length transcriptome, and Illumina RNA-seq to compare 4-year cultivated ginseng with 26-year wild-simulated ginseng. After clustering and error correction, we obtained 265,373 (CG) and 210,343 (WSG) non-redundant full-length transcript isoforms. Total saponins ranked leaves > roots > stems. In leaves, PPT type (Re and Rg1) predominated and was significantly higher in CG than WSG; in roots, PPD type dominated, and all monomer saponins in WSG were significantly higher. Transcriptome analysis revealed widespread alternative splicing (mainly retained introns), with key genes like

Indexed as

alternative splicingfull-length transcriptomeginsenosideWGCNAwild-simulated ginseng

Identifiers

PMID42651673

DOI10.3390/biology15161367

PMCPMC13509296

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.