Evidence map›Paper›PMID 42650884›Full record

ArticleBiomolecules2026

Fluorescent Analysis of K

Vladislava Martyshova, Sergey Sedykh

Abstract read
In one paragraph

Article in Biomolecules, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Vladislava MartyshovaFaculty of Natural Sciences, School of Advanced Engineering Studies, Novosibirsk State University, Lyapunova St., 1, 630090 Novosibirsk, Russia.
Sergey SedykhFaculty of Natural Sciences, School of Advanced Engineering Studies, Novosibirsk State University, Lyapunova St., 1, 630090 Novosibirsk, Russia.ORCID 0000-0003-0882-8171

Funding

The Ministry of Education and Science of the Russian Federation 075-15-2025-473
6 · The paper itself

Abstract

Methyl-dependent restriction endonucleases are promising tools for analyzing eukaryotic DNA methylation patterns. However, quantitative assessment of their substrate specificity requires the determination of the kinetic parameters of enzymatic reactions. Here, we present a method for determining initial reaction rates based on fluorescent probes and real-time monitoring of changes in fluorescence intensity. Initial rates of methyl-dependent

Indexed as

Deoxyribonucleases, Type II Site-SpecificDNA Restriction EnzymesFluorescent DyesDNA MethylationFluorescenceKineticsSpectrometry, FluorescenceSubstrate SpecificityDeoxyribonucleases, Type II Site-SpecificDNA Restriction EnzymesFluorescent DyesDNA methylationenzyme kineticsFRETMichaelis–Menten constantreaction raterestriction endonucleases

Identifiers

PMID42650884
PMCPMC13510852

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.