Evidence map›Paper›PMID 42650696›Full record

ArticleAntibiotics (Basel, Switzerland)2026

Molecular Characterization of the

Jacqueline Samuel Ulomi, Peter M Mbelele, Jonas Ngowo, David Mtweve, Helena Dela, Bruno Enagnon Lokonon, Bassirou Bonfoh, Esther G Kimaro, Beatus Lyimo

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Antibiotics (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jacqueline Samuel UlomiDepartment of Global Health and Biomedical Sciences, School of Life Sciences, Nelson Mandela African Institution of Science and Technology, Arusha P.O. Box 447, Tanzania.ORCID 0009-0000-0959-566X
Peter M MbeleleDepartment of Global Health and Biomedical Sciences, School of Life Sciences, Nelson Mandela African Institution of Science and Technology, Arusha P.O. Box 447, Tanzania.ORCID 0000-0002-2693-187X
Jonas NgowoDepartment of Global Health and Biomedical Sciences, School of Life Sciences, Nelson Mandela African Institution of Science and Technology, Arusha P.O. Box 447, Tanzania.
David MtweveDepartment of Global Health and Biomedical Sciences, School of Life Sciences, Nelson Mandela African Institution of Science and Technology, Arusha P.O. Box 447, Tanzania.
Helena DelaNoguchi Memorial Institute for Medical Research, Legon, Accra P.O. Box LG 581, Ghana.ORCID 0000-0001-8118-9965
Bruno Enagnon LokononCentre Suisse de Recherches Scientifiques en Côte d'Ivoire, Abidjan 01 BP 1303, Côte d'Ivoire.ORCID 0000-0002-0574-8793
Bassirou BonfohCentre Suisse de Recherches Scientifiques en Côte d'Ivoire, Abidjan 01 BP 1303, Côte d'Ivoire.ORCID 0000-0003-4190-8202
Esther G KimaroDepartment of Global Health and Biomedical Sciences, School of Life Sciences, Nelson Mandela African Institution of Science and Technology, Arusha P.O. Box 447, Tanzania.
Beatus LyimoDepartment of Global Health and Biomedical Sciences, School of Life Sciences, Nelson Mandela African Institution of Science and Technology, Arusha P.O. Box 447, Tanzania.ORCID 0000-0002-6743-4546

Funding

Afrique One - REACH Not ApplicableWellcome Trust
6 · The paper itself

Abstract

BACKGROUND/

objectivesZoonotic tuberculosis (TB) remains a persistent public health challenge worldwide. It is particularly common in settings with close human-livestock-environment interactions. In Tanzania, progress toward TB control is increasingly threatened by multidrug-resistant tuberculosis (MDR-TB), yet genomic data from regions characterized by pastoralist and mining activities remain scarce. This study employed whole-genome sequencing (WGS) to characterize

methodsThis cross-sectional study was conducted between September 2024 and February 2025. A total of 178 presumptive human TB cases provided sputum samples. From cattle, 161 samples were collected (110 milk samples and 51 lymph node aspirates), with each animal contributing only one type of sample. Specimen were analyzed using GeneXpert MTB/RIF, Lowenstein-Jensen culture, and WGS. Phylogenetic reconstruction was performed using SNP-based methods and IQ-TREE2 version 2.2.0.

resultsAmong human participants, 14 (7.8%) sputum samples were GeneXpert positive and were confirmed as members of MTBC by LJ culture. In cattle, one (0.62%) lymph node aspirate was positive for MTBC. Significant predictors of MTBC positivity included previous TB history, weight loss, and occupation involving mining and cattle keeping. WGS of five human isolates identified Lineages 1, 3, and 4. One isolate (Sample 98) harbored mutations associated with XDR-TB.

conclusionsWGS revealed

Indexed as

lineagesManyaraM. tuberculosis complex (MTBC)phylogenetic analysiswhole-genome sequencing

Identifiers

PMID42650696
PMCPMC13509724

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.