ArticleScientific data2026
Four haplotype-resolved genome assemblies and a reference-free 66-haplotype pangenome graph for Cannabis sativa.
Article in Scientific data, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Four haplotype-resolved genome assemblies and a reference-free 66-haplotype pangenome graph for Cannabis sativa.Scientific data · 2026Article
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3 authors.
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Abstract
We present 4 haplotype-resolved, chromosome scale diploid assemblies of cannabis, assembled from ONT R9.4.1 reads via a novel pipeline based on Hi-C phasing. These assemblies, while low in QV, offer contiguity and genic content comparable to recent HiFi assemblies. Along with a trio-binned assembly previously produced by us and 56 haplotypes selected from the Salk Institute Cannabis Pangenome project, we use these assemblies to create a reference-free pangenome graph. Within a total length of 6.48 Gb, it contains 162.14 M nodes, 228.27 M edges, 14.87 M SNPs, and 6.40 M indels. By optimizing parameters within the Pangenome Graph Builder (PGGB), we avoid many spurious connections among repeat elements, reduce processing time, and arrive at a data structure that visibly recapitulates the linear nature of plant chromosomes. Via k-mer analysis, we corroborate that more genotypes are needed to close the cannabis pangenome, and that, in particular, the region of origin likely remains undersampled.
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