Evidence map›Paper›PMID 42648361›Full record

ArticleThe Journal of biological chemistry2026

Use of high-affinity scFv antibody NUsc1 for isolation and sensitive detection of Alzheimer's-associated amyloid beta oligomers.

Raquel Maria de Campos, Mariane Fávero Carraro, André Luiz Brandão Bitencourt, Joshua J Jerisha, Navid Jubaer Ayon, Vuslat B Juska, Nathalia Reges Pinheiro, Elizabeth A Johnson, Raghad N Nowar, John P McGee and 14 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

24 authors.

Raquel Maria de CamposDepartment of Neurobiology, Northwestern University, Evanston, Illinois, USA; Department of Biochemistry and Immunology, Ribeirao Preto Medical School, University of São Paulo, São Paulo, Brazil. Electronic address: raquel.decampos@northwestern.edu.
Mariane Fávero CarraroDepartment of Biochemistry and Immunology, Ribeirao Preto Medical School, University of São Paulo, São Paulo, Brazil.
André Luiz Brandão BitencourtDepartment of Biochemistry and Immunology, Ribeirao Preto Medical School, University of São Paulo, São Paulo, Brazil.
Joshua J JerishaDepartment of Neurobiology, Northwestern University, Evanston, Illinois, USA.
Navid Jubaer AyonDepartments of Chemistry and Molecular Biosciences, Department of Chemical and Biological Engineering, the Chemistry of Life Processes Institute, The Proteomics Center of Excellence at Northwestern University, Evanston, Illinois, USA.
Vuslat B JuskaDepartment of Chemistry, Weinberg College of Arts & Sciences, Northwestern University, Evanston, Illinois, USA.
Nathalia Reges PinheiroDepartment of Biochemistry and Immunology, Ribeirao Preto Medical School, University of São Paulo, São Paulo, Brazil.
Elizabeth A JohnsonDepartment of Chemistry, Weinberg College of Arts & Sciences, Northwestern University, Evanston, Illinois, USA.
Raghad N NowarDepartment of Neurobiology, Northwestern University, Evanston, Illinois, USA.
John P McGeeDepartments of Chemistry and Molecular Biosciences, Department of Chemical and Biological Engineering, the Chemistry of Life Processes Institute, The Proteomics Center of Excellence at Northwestern University, Evanston, Illinois, USA.
Mauro Exequiel AlfaroDepartment of Neurobiology, Northwestern University, Evanston, Illinois, USA.
Maria Victoria OberholzerInstituto de Biología Celular y Neurociencia (IBCN), Universidad de Buenos Aires (UBA) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Autónoma de Buenos Aires, Argentina.
Vanina GrippoInstituto de Ciencia y Tecnología "Dr César Milstein" (ICT-Milstein), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) - Fundación Pablo Cassará, Ciudad Autónoma de Buenos Aires, Argentina.
Erika N ClineDepartment of Chemistry, Weinberg College of Arts & Sciences, Northwestern University, Evanston, Illinois, USA.
Kirsten L ViolaDepartment of Neurobiology, Northwestern University, Evanston, Illinois, USA.
Neil L KelleherDepartments of Chemistry and Molecular Biosciences, Department of Chemical and Biological Engineering, the Chemistry of Life Processes Institute, The Proteomics Center of Excellence at Northwestern University, Evanston, Illinois, USA.
Martin HabifDepartment of Neurobiology, Northwestern University, Evanston, Illinois, USA.
Diana Alicia JerusalinskyInstituto de Biología Celular y Neurociencia (IBCN), Universidad de Buenos Aires (UBA) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Autónoma de Buenos Aires, Argentina.
Gajendra S ShekhawatInternational Institute for Nanotechnology and NUANCE Center, Department of Materials Science and Engineering, Northwestern University, Evanston, Illinois, USA.
Morgan B ElmorDepartment of Neurology and Goizueta Alzheimer's Disease Research Center, Emory University, Atlanta, Georgia, USA.
James J LahDepartment of Neurology and Goizueta Alzheimer's Disease Research Center, Emory University, Atlanta, Georgia, USA.
Allan I LeveyDepartment of Neurology and Goizueta Alzheimer's Disease Research Center, Emory University, Atlanta, Georgia, USA.
William L KleinDepartment of Neurobiology, Northwestern University, Evanston, Illinois, USA.
Adriano SebollelaDepartment of Biochemistry and Immunology, Ribeirao Preto Medical School, University of São Paulo, São Paulo, Brazil.

Funding

Tumor Environment and Metastasis (TEAM) Research ProgramP30CA060553 · NCI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI Devalingam Mahalingam · 1993 to 2026
$153.9M
Novel Protein Aggregation Inhibitors and Upper Motor Neuron Stabilizers for ALS and other Neurodegenerative DiseasesR01AG061708 · NIA · NORTHWESTERN UNIVERSITY AT CHICAGO · PI WILLIAM L KLEIN, Pembe Hande Ozdinler · 2019 to 2026
$10.5M
NCI NIH HHS P30 CA060553NIA NIH HHS R01 AG061708
6 · The paper itself

Abstract

Alzheimer's disease (AD) is a progressive neurodegenerative disorder in which soluble amyloid-beta oligomers (AβOs) play a key role in disease onset and progression. This study further explores NUsc1, a single-chain variable fragment (scFv) antibody with high specificity for neurotoxic AβOs over monomers or fibrils, to selectively isolate and assist characterization of AβO species. NUsc1 binds AβOs with a kinetic dissociation constant under 10 nM, comparable to the affinity of Leqembi for protofibrils. NUsc1 inhibits aggregation of AβOs into protofibrillar and fibrillar structures and blocks AβO binding to cultured hippocampal neurons in a dose-dependent manner, with an IC

Indexed as

Alzheimer's diseaseamyloid β oligomersconformational antibodiesELISANUsc1scFv

Identifiers

PMID42648361
PMCPMC13631620

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.