Evidence map›Paper›PMID 42647848›Full record

ArticleEmerging microbes & infections2026

Molecular cloning of a monkeypox virus clade IIb genome and construction of an attenuated mutant for basic and translational research.

Vincent Klatt, Christian Meyer Zu Natrup, Laura Staliunaite, Sabrina Clever, Jan Knickmann, Sonja Ohrnberger, Jan Seib, Eleonore Ostermann, Hanna-Marie Weichel, Marylyn M Addo and 2 more

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Article in Emerging microbes & infections, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Vincent KlattLeibniz Institute of Virology (LIV), Hamburg, Germany.
Christian Meyer Zu NatrupInstitute of Virology, University of Veterinary Medicine Hannover, Hannover, Germany.
Laura StaliunaiteLeibniz Institute of Virology (LIV), Hamburg, Germany.
Sabrina CleverInstitute of Virology, University of Veterinary Medicine Hannover, Hannover, Germany.
Jan KnickmannLeibniz Institute of Virology (LIV), Hamburg, Germany.
Sonja OhrnbergerInstitute of Virology, University of Veterinary Medicine Hannover, Hannover, Germany.
Jan SeibInstitute of Virology, University of Veterinary Medicine Hannover, Hannover, Germany.
Eleonore OstermannLeibniz Institute of Virology (LIV), Hamburg, Germany.
Hanna-Marie WeichelGerman Center for Infection Research (DZIF), Partner Site Hamburg-Lübeck-Borstel-Riems, Hamburg, Germany.
Marylyn M AddoGerman Center for Infection Research (DZIF), Partner Site Hamburg-Lübeck-Borstel-Riems, Hamburg, Germany.
Asisa VolzInstitute of Virology, University of Veterinary Medicine Hannover, Hannover, Germany.
Wolfram BruneLeibniz Institute of Virology (LIV), Hamburg, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Monkeypox virus (MPXV) is a rodent-borne orthopoxvirus causing mpox disease upon zoonotic transmission to humans. Recent global outbreaks have raised awareness and highlighted the need for basic and translational research. However, research is hampered by the classification of MPXV as a Risk Group 3 pathogen. In an effort to overcome this limitation, we generated a complete full-length MPXV clade IIb bacterial artificial chromosome (BAC) clone by single-step transformation-associated recombination (STAR) cloning in yeast. The integrity of the cloned MPXV genome sequence and the replication capability of the BAC-derived virus were verified. To obtain an attenuated mutant, the viral thymidine kinase (TK) gene,

Indexed as

Genome, ViralMonkeypox virusMpox, MonkeypoxAnimalsChromosomes, Artificial, BacterialCloning, MolecularHumansMiceMutationThymidine KinaseVirus ReplicationThymidine KinaseattenuationMpoxneutralization testSTAR cloningthymidine kinase

Identifiers

PMID42647848
PMCPMC13520863

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.