Evidence map›Paper›PMID 42647375›Full record

ArticleProteomes2026

Proteomic Analysis of Paraffin-Embedded Intestines from

Lara Geralda Magela Dos Santos Vieira, Ana Flávia Pinho Souza, Camilo Elber Vital, Dávila Regina Pacheco Silva, Flávia de Souza Marques, Gustavo Gonçalves Silva, Paula Melo de Abreu Vieira, R Alan Wilson, William Castro-Borges

Abstract read
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In one paragraph

Article in Proteomes, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Lara Geralda Magela Dos Santos VieiraPrograma de Pós-Graduação em Ciências Biológicas, Núcleo de Pesquisas em Ciências Biológicas, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto 35402-136, Minas Gerais, Brazil.
Ana Flávia Pinho SouzaPrograma de Pós-Graduação em Ciências Biológicas, Núcleo de Pesquisas em Ciências Biológicas, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto 35402-136, Minas Gerais, Brazil.
Camilo Elber VitalNúcleo de Pesquisas em Ciências Biológicas, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto 35402-136, Minas Gerais, Brazil.
Dávila Regina Pacheco SilvaPrograma de Pós-Graduação em Biotecnologia, Núcleo de Pesquisas em Ciências Biológicas, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto 35402-136, Minas Gerais, Brazil.ORCID 0000-0002-1318-1512
Flávia de Souza MarquesPrograma de Pós-Graduação em Ciências Biológicas, Núcleo de Pesquisas em Ciências Biológicas, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto 35402-136, Minas Gerais, Brazil.
Gustavo Gonçalves SilvaPrograma de Pós-Graduação em Ciências Biológicas, Núcleo de Pesquisas em Ciências Biológicas, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto 35402-136, Minas Gerais, Brazil.
Paula Melo de Abreu VieiraDepartamento de Ciências Biológicas, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto 35402-136, Minas Gerais, Brazil.ORCID 0000-0001-7033-7686
R Alan WilsonDepartment of Biology, York Biomedical Research Institute, University of York, York YO10 5DD, UK.ORCID 0000-0002-7210-0321
William Castro-BorgesDepartamento de Ciências Biológicas, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto 35402-136, Minas Gerais, Brazil.

Funding

Coordenação de Aperfeicoamento de Pessoal de Nível Superior 001Fundação de Amparo à Pesquisa do Estado de Minas Gerais APQ-00067-23Fundação de Amparo à Pesquisa do Estado de Minas Gerais APQ-00782-22Fundação de Amparo à Pesquisa do Estado de Minas Gerais APQ-01703-24Fundação de Amparo à Pesquisa do Estado de Minas Gerais APQ - 02342-23Fundação de Amparo à Pesquisa do Estado de Minas Gerais APQ-02511-22National Council for Scientific and Technological Development 311532/2022-5
6 · The paper itself

Abstract

backgroundAdult

methodsHere, we investigate parasite-host interactions in a mouse model during the acute phase of a patent infection at 5 and 7 weeks, using parallel histological and proteomic analysis of paraffin-embedded ileal tissues.

resultsHistology at week 7 showed infected animals had more inflammation and longer villi than at week 5, as well as more Goblet cells reflecting enhanced mucus production. LC-MS/MS analysis of deparaffinized ileal sections, subjected to in-solution tryptic digestion, revealed a total of 1615 protein groups. Differentially abundant proteins were found early at week 5, coinciding with the onset of egg deposition. A contrasting scenario, dominated by upregulation of protein components from the innate and adaptive immune systems, was seen at week 7; at this point, egg migration and excretion are underway. Among the proteins were mast cell proteases, fibrinogens, arginase-1, and molecules associated with extracellular matrix remodeling.

conclusionsOur findings reflect intestinal proteome changes likely participating in

Indexed as

intestinesparaffin-embeddedproteomic analysisSchistosoma mansoni

Identifiers

PMID42647375
PMCPMC13510969

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.