Evidence map›Paper›PMID 42647171›Full record

ArticleSTAR protocols2026

Protocol for 3D digital dynamic histomorphometry of mouse bone via time-lapse registration of serial microCT scans.

Quentin A Meslier, Nicole Migotsky, Syeda N Lamia, Erica L Scheller, Matthew J Silva

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Quentin A MeslierBone and Mineral Disease Division, Department of Medicine, School of Medicine, Washington University in St. Louis, St. Louis, MO, USA; Center of Regenerative Medicine, School of Medicine, Washington University in St. Louis, St. Louis, MO, USA.
Nicole MigotskyDepartment of Orthopedic Surgery, School of Medicine, Washington University in St. Louis, St. Louis, MO, USA.
Syeda N LamiaDepartment of Orthopedic Surgery, School of Medicine, Washington University in St. Louis, St. Louis, MO, USA.
Erica L SchellerBone and Mineral Disease Division, Department of Medicine, School of Medicine, Washington University in St. Louis, St. Louis, MO, USA; Center of Regenerative Medicine, School of Medicine, Washington University in St. Louis, St. Louis, MO, USA; Department of Biomedical Engineering, McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, USA.
Matthew J SilvaDepartment of Orthopedic Surgery, School of Medicine, Washington University in St. Louis, St. Louis, MO, USA; Department of Biomedical Engineering, McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, USA. Electronic address: silvam@wustl.edu.

Funding

Response of the Osteoporotic Skeleton to Mechanical LoadingR01AR047867 · NIAMS · WASHINGTON UNIVERSITY · PI MATTHEW J SILVA · 2001 to 2026
$8.4M
Resource Based Center for Musculoskeletal Biology and Medicine (Overall Application)P30AR074992 · NIAMS · WASHINGTON UNIVERSITY · PI MATTHEW J SILVA · 2019 to 2026
$6.8M
Sarm1 and neural regulation of boneR01DK132073 · NIDDK · WASHINGTON UNIVERSITY · PI Erica Lynn Scheller · 2023 to 2026
$1.6M
Influence of Genetic Background on Bone Anabolic Response to Mechanical LoadingR21AR079052 · NIAMS · WASHINGTON UNIVERSITY · PI SILVA, MATTHEW J · 2022 to 2023
$394k
NIAMS NIH HHS P30 AR074992NIAMS NIH HHS R01 AR047867NIAMS NIH HHS R21 AR079052NIDDK NIH HHS R01 DK132073
6 · The paper itself

Abstract

Serial in vivo microCT enables the quantification of dynamic bone remodeling by capturing formation and resorption over time. Here, we present a protocol to register pre- and post-intervention scans of rodent long bones through an intuitive drag-and-click workflow. We describe steps for rigid registration, enabling spatial mapping and quantification of formed, resorbed, and quiescent cortical and trabecular bone at periosteal and endosteal surfaces across multiple tibial regions. This protocol provides a non-destructive and complementary alternative to classic, fluorochrome-based dynamic histomorphometry.

Indexed as

BiophysicsBiotechnology and bioengineeringStructural biology

Identifiers

PMID42647171
PMCPMC13543904

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.