Optimisation and Validation of a Microscale FRAP Assay with Matrix-Matched (Interaction-Corrected) Blanking for Accurate Antioxidant Capacity Assessment of Honeys.

Ivan Lozada Lawag, Sharmin Sultana, Lee Yong Lim, Cornelia Locher

Abstract read

In one paragraph

Article in Methods and protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Ivan Lozada LawagDepartment of Pharmacy and Centre for Optimisation of Medicines, School of Health and Clinical Sciences, University of Western Australia, Crawley, Perth, WA 6009, Australia.ORCID 0000-0002-8805-3697

Sharmin SultanaDepartment of Pharmacy and Centre for Optimisation of Medicines, School of Health and Clinical Sciences, University of Western Australia, Crawley, Perth, WA 6009, Australia.

Lee Yong LimDepartment of Pharmacy and Centre for Optimisation of Medicines, School of Health and Clinical Sciences, University of Western Australia, Crawley, Perth, WA 6009, Australia.ORCID 0000-0002-7307-0742

Cornelia LocherDepartment of Pharmacy and Centre for Optimisation of Medicines, School of Health and Clinical Sciences, University of Western Australia, Crawley, Perth, WA 6009, Australia.ORCID 0000-0001-8773-3036

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study optimised and validated a microplate Ferric-Reducing Antioxidant Power (FRAP) assay for honey that incorporates a honey-specific matrix-matched blank-a paired absorbance measurement lacking the chromogenic probe 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ)-to separate true ferric reduction from non-reductive Fe

Indexed as

assay optimisation and validationferric-reducing antioxidant power (FRAP) assayflavonoidshoneyiron–polyphenol interactionsmatrix effectsmatrix-matched blankingphenolic acidsTPTZUV–Vis spectrophotometry

Identifiers

PMID42646515

DOI10.3390/mps9040120

PMCPMC13515261

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.