ArticleApplied microbiology and biotechnology2026
Efficient biotechnological production of N-(Hydroxycinnamoyl)tyramines by utilizing plant-derived enzymes in a bench-scale bioreactor with in situ product precipitation.
Article in Applied microbiology and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.
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9 authors.
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Abstract
The efficient recovery of plant polyphenolic bioactives is facing technological challenges of low concentrations and complex mixtures starting from plant tissue. In the search of alternatives, we developed a high-yield biotechnological production process for hydroxycinnamic acid-tyramine conjugates in Escherichia coli by converting soluble substrates into insoluble products. A recombinant plant-enzyme cascade was established by co-expression of Arabidopsis thaliana 4-coumarate:CoA ligase and Solanum tuberosum hydroxycinnamoyl-CoA:tyramine N-(hydroxycinnamoyl)transferase. We overcame initial enzymatic limitations by developing a temperature adapted and sequentially phased bioprocess on a 1 L reactor scale. With 5 mM/h substrate supply rate we achieved 31.9 g/L (102 mM) of feruloyltyramine (FerT), 14.4 g/L (50.7 mM) coumaroyltyramine, and 14.6 g/L (46.5 mM) isoferuloyltyramine with a space time yield of 1.75 g *L
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