ArticleFrontiers in microbiology2026
Genome-resolved insights into hydrocarbon-transforming and nitrate-reducing microbial communities from deep petroleum reservoir cores of the Nashpa Oil Field, Pakistan.
Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: Microbial communities from deep subsurface petroleum reservoirs are adapted to hydrocarbon-rich, oxygen-limited, and physicochemically extreme environments. However, genome-resolved knowledge of petroleum reservoir microbiomes from Pakistan remains largely unexplored. Methods: Shotgun metagenomic sequencing was used to investigate the functional and metabolic potential of microbial communities inhabiting deep subsurface petroleum reservoir cores from the Nashpa Oil Field, Pakistan, at depths of 3,770-4,315 m. Metagenome-assembled genomes (MAGs) were reconstructed and functionally annotated to assess taxonomic composition and predicted metabolic capabilities. Results: A total of 402 metagenome-assembled genomes (MAGs) were recovered, of which 216 were high-quality MAGs (≥90% completeness and ≤5% contamination). Taxonomic analysis showed dominance of Discussion: This study provides one of the first genome-resolved insights into deep petroleum reservoir microbiomes from Pakistan and identifies candidate microbial lineages carrying MEOR-relevant genomic traits for future functional validation.
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