Evidence map›Paper›PMID 42643426›Full record

ArticleFrontiers in veterinary science2026

Development of a dual fluorescent microsphere-based lateral flow immunoassays for simultaneous detection of

Dihui Meng, Rongsheng Mi, Wei Zhu, Ying Zhang, Ke Lu, Zhaoguo Chen, Xiangan Han, Quan Wang, Jin Ni, Jun Wu and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in veterinary science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Dihui Meng *College of Food Science and Engineering, Jilin Agricultural University, Changchun, China.
Rongsheng Mi *Key Laboratory of Animal Parasitology of Ministry of Agriculture, Laboratory of Quality and Safety Risk Assessment for Animal Products on Biohazards (Shanghai) of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Wei ZhuTengzhou Animal Disease Prevention and Control Center of Shandong Province, Tengzhou, China.
Ying ZhangJinhua Key Laboratory of Genetic Breeding and Improvement on Jinhua Pig, College of Agriculture, Jinhua University of Vocational Technology, Jinhua, China.
Ke LuKey Laboratory of Animal Parasitology of Ministry of Agriculture, Laboratory of Quality and Safety Risk Assessment for Animal Products on Biohazards (Shanghai) of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Zhaoguo ChenKey Laboratory of Animal Parasitology of Ministry of Agriculture, Laboratory of Quality and Safety Risk Assessment for Animal Products on Biohazards (Shanghai) of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Xiangan HanKey Laboratory of Animal Parasitology of Ministry of Agriculture, Laboratory of Quality and Safety Risk Assessment for Animal Products on Biohazards (Shanghai) of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Quan WangKey Laboratory of Animal Parasitology of Ministry of Agriculture, Laboratory of Quality and Safety Risk Assessment for Animal Products on Biohazards (Shanghai) of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Jin NiCollege of Food Science and Engineering, Jilin Agricultural University, Changchun, China.
Jun WuCollege of Food Science and Engineering, Jilin Agricultural University, Changchun, China.
Baoping GuoState Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, and WHO-Collaborating Centre for Prevention and Care Management of Echinococcosis, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.
Lixin YouCollege of Food Science and Engineering, Jilin Agricultural University, Changchun, China.
Guodong MuJilin Center for Animal Disease Control and Prevention, Changchun, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Bovine brucellosis and bovine tuberculosis are important zoonotic diseases that pose serious threats to human and animal health and cause substantial economic losses in the livestock industry. Therefore, a rapid diagnostic method suitable for the simultaneous field detection of both diseases is urgently needed. Methods: Fluorescent microspheres conjugated with recombinant streptococcal protein G (rSPG) were prepared and used as tracers. Results and discussion: The dual rapid test strip enabled detection to be completed within 20 min. The optimal coating concentrations were determined to be 0.5 mg/mL for the Brucella test line (TL1) and 1.0 mg/mL for the

Indexed as

Brucella spp.immunochromatographic striplipopolysaccharide (LPS)MPB70Mycobacterium bovis

Identifiers

PMID42643426
PMCPMC13503215

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.