Evidence map›Paper›PMID 42642739›Full record

ArticleEpigenetics & chromatin2026

Drosophila Rumpelstiltskin regulates Polycomb cis and trans interactions and Homie barrier activity.

Savanna F Lyda, Catherine E McManus, Juan Manuel Caravaca, Dagyeong Yang, Yuejun Wang, Hernan A Lorenzi, Fedor Kouzine, David Levens, Leah F Rosin, Elissa P Lei

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Article in Epigenetics & chromatin, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Savanna F LydaNuclear Organization and Gene Expression Section, Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.
Catherine E McManusNuclear Organization and Gene Expression Section, Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.
Juan Manuel CaravacaNuclear Organization and Gene Expression Section, Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.
Dagyeong YangNuclear Organization and Gene Expression Section, Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.
Yuejun WangTriLab Bioinformatics Group, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.
Hernan A LorenziTriLab Bioinformatics Group, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.
Fedor KouzineGene Regulation Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
David LevensGene Regulation Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Leah F RosinNuclear Organization and Gene Expression Section, Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.
Elissa P LeiNuclear Organization and Gene Expression Section, Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA. leielissa@niddk.nih.gov.

Funding

NIDDK NIH HHS DK015602
6 · The paper itself

Abstract

backgroundThree-dimensional genome organization is maintained in part by chromatin insulators, DNA-protein complexes that create boundaries between active and inactive chromatin and regulate enhancer-promoter interactions. In Drosophila, the Homing insulator at eve (Homie) sequence prevents repressive Polycomb (PcG) chromatin at even-skipped (eve) from spreading into the neighboring essential gene, TER94. The exact mechanisms controlling Homie barrier activity are understudied.

resultsHere, use of an in vivo reporter assay identified the requirement of the hnRNP M homolog, Rumpelstiltskin (Rump), for Homie barrier activity. Interestingly, Rump depletion resulted in no changes to chromatin insulator protein binding at Homie but extensive chromatin association changes to the Polycomb response element (PRE)-associated protein Crooked legs (Crol) at Crol-bound PREs. Furthermore, global chromatin association changes were detected for a subset of Polycomb repressive complex 1 and 2 (PRC1 and PRC2) components. Finally, we observed increased cis-compaction at Polycomb domains genome-wide including eve and increased distances between eve and other Polycomb domains in trans after Rump depletion.

conclusionsThese data identify Rump as a novel regulator of Homie barrier activity and suggest a regulatory role of Rump for both cis and trans interactions of PcG proteins. Importantly, this regulatory role likely helps to maintain Homie barrier activity independently of chromatin insulator proteins.

Indexed as

Drosophila melanogasterDrosophila ProteinsInsulator ElementsPolycomb-Group ProteinsAnimalsChromatinProtein BindingChromatinDrosophila ProteinsPolycomb-Group Proteins3D genome organizationChromatin insulatorHomiePolycombPRE

Identifiers

PMID42642739
PMCPMC13504762

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