Isolation of RNA and MicroRNAs from Formalin-Fixed, Paraffin-Embedded Tissues (FFPE), and Cultured Cells for Subsequent Applications.

Sofia Marotta, Stella Mrmić, Lucia Mundo, Tommaso Salamone, Eugenio Santacroce, Pankaj Trivedi, Eleni Anastasiadou

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Sofia Marotta *Department of Clinical and Molecular Medicine, Sapienza University, Rome, Italy.

Stella Mrmić *Department of Clinical and Molecular Medicine, Sapienza University, Rome, Italy.

Lucia Mundo *Department of Clinical and Molecular Medicine, Sapienza University, Rome, Italy.

Tommaso SalamoneDepartment of Clinical and Molecular Medicine, Sapienza University, Rome, Italy.

Eugenio SantacroceDepartment of Clinical and Molecular Medicine, Sapienza University, Rome, Italy.

Pankaj TrivediDepartment of Experimental Medicine, Sapienza University, Rome, Italy. pankaj.trivedi@uniroma1.it.

Eleni AnastasiadouDepartment of Clinical and Molecular Medicine, Sapienza University, Rome, Italy. eleni.anastasiadou@uniroma1.it.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

MicroRNAs (miRNAs) are small, non-coding RNAs that play critical roles in post-transcriptional gene regulation and are involved in various biological processes, including cancer progression. Efficient extraction of miRNAs from cultured cells and tissue samples is crucial for downstream applications, including quantitative real-time PCR (qRT-PCR), next-generation sequencing (NGS), and microarray analysis. This chapter provides reliable protocols for extracting high-quality RNA and microRNAs from formalin-fixed paraffin-embedded (FFPE) tissues and cultured cells. It compares manual and automated methods to guide optimal kit selection. The protocols support reproducible results from challenging samples in research and clinical settings.

Indexed as

MicroRNAsParaffin EmbeddingRNATissue FixationCells, CulturedFormaldehydeHigh-Throughput Nucleotide SequencingHumansReal-Time Polymerase Chain ReactionFormaldehydeMicroRNAsRNABioanalyzerCancer cell linesFFPE tissuesmiRNA isolationQubit fluorometerRNA extractionRNA&miRNA integrityRNase contamination preventionRNA stabilizationTapeStation

Identifiers

PMID42642600

DOI10.1371/journal.pone.0257070

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.