Single Molecule Tracking: Visualizing Transcription of Endogenous Loci in Living Cells.

Pelin Yaşar, Christopher R Day, Joseph Rodriguez

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Pelin YaşarEpigenetics and RNA Biology Laboratory, National Institute of Environmental Health Sciences (NIEHS), Research Triangle Park, Durham, NC, USA.

Christopher R DayEpigenetics and RNA Biology Laboratory, National Institute of Environmental Health Sciences (NIEHS), Research Triangle Park, Durham, NC, USA.

Joseph RodriguezEpigenetics and RNA Biology Laboratory, National Institute of Environmental Health Sciences (NIEHS), Research Triangle Park, Durham, NC, USA. joseph.rodriguez@nih.gov.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Live-cell imaging of RNA provides mechanistic insights into the spatiotemporal organization and regulation of transcription. The most widely used method for visualizing transcription in living cells is the MS2 aptamer system. During nascent RNA synthesis of an MS2-labeled gene, a transcribed array of MS2 RNA stem-loops is bound by fluorescently labeled MS2 coat proteins to enable the visualization and tracking of single RNA molecules. Here, we present a protocol for visualizing nascent RNA transcription at an endogenous gene locus. Given the importance of the native chromatin context in regulating transcription, using this approach is crucial for uncovering mechanisms and understanding gene expression dynamics.

Indexed as

Genetic LociRNASingle Molecule ImagingTranscription, GeneticChromatinHumansLevivirusMicroscopy, FluorescenceSingle-Cell AnalysisSingle-Cell Gene Expression AnalysisChromatinRNAMS2Single-cellSingle-molecule trackingTranscription

Identifiers

PMID42642595

DOI10.1021/jp903831z

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.