Evidence map›Paper›PMID 42640357›Full record

ArticleTranslational stroke research2026

Periodontal Bacteria Proteomics in Thrombi from Ischemic Stroke Patients.

Florencia Gayo, Susana Bravo, Jorge Moldes, Carmen Pena, Manuel Rodríguez-Yáñez, Rafael Domínguez-Cachón, Ramón Iglesias-Rey, Antonio Jesús Mosqueira-Martínez, Juan Blanco, Tomás Sobrino and 1 more

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Article in Translational stroke research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Florencia GayoUnit of Periodontology, Faculty of Dentistry and Medicine, University of Santiago de Compostela and Medical-Surgical Dentistry Research Group (OMEQUI), Health Research Institute of Santiago de Compostela (IDIS), Rúa Entrerríos S/N, Santiago de Compostela, 15782, Spain.
Susana BravoProteomic Unit, Health Research Institute of Santiago de Compostela, University Clinical Hospital, Santiago de Compostela, Spain.
Jorge MoldesUnit of Periodontology, Faculty of Dentistry and Medicine, University of Santiago de Compostela and Medical-Surgical Dentistry Research Group (OMEQUI), Health Research Institute of Santiago de Compostela (IDIS), Rúa Entrerríos S/N, Santiago de Compostela, 15782, Spain.
Carmen PenaProteomic Unit, Health Research Institute of Santiago de Compostela, University Clinical Hospital, Santiago de Compostela, Spain.
Manuel Rodríguez-YáñezStroke Unit, Neurology Department, University Clinical Hospital, Santiago de Compostela, Spain.
Rafael Domínguez-CachónUnit of Periodontology, Faculty of Dentistry and Medicine, University of Santiago de Compostela and Medical-Surgical Dentistry Research Group (OMEQUI), Health Research Institute of Santiago de Compostela (IDIS), Rúa Entrerríos S/N, Santiago de Compostela, 15782, Spain.
Ramón Iglesias-ReyNeuroimaging and Biotechnology Laboratory Group, Clinical Neurosciences Research Laboratories, Health Research Institute of Santiago de Compostela, University Clinical Hospital, Santiago de Compostela, Spain.
Antonio Jesús Mosqueira-MartínezRadiology Department, University Clinical Hospital, Santiago de Compostela, Spain.
Juan BlancoUnit of Periodontology, Faculty of Dentistry and Medicine, University of Santiago de Compostela and Medical-Surgical Dentistry Research Group (OMEQUI), Health Research Institute of Santiago de Compostela (IDIS), Rúa Entrerríos S/N, Santiago de Compostela, 15782, Spain.
Tomás Sobrino *NeuroAging Group (NEURAL), Clinical Neurosciences Research Laboratory (LINC), Health Research Institute of Santiago de Compostela (IDIS), Santiago de Compostela, Spain.
Yago Leira *Unit of Periodontology, Faculty of Dentistry and Medicine, University of Santiago de Compostela and Medical-Surgical Dentistry Research Group (OMEQUI), Health Research Institute of Santiago de Compostela (IDIS), Rúa Entrerríos S/N, Santiago de Compostela, 15782, Spain. yago.leira.feijoo@usc.es.

Funding

Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas CB22/05/00067European Union's Horizon 2020 research and innovation program (Marie Skłodowska-Curie grant agreement ) No. 101066444Instituto de Salud Carlos III PI22/00938Instituto de Salud Carlos III, Spain CP22/00061INTERREG Atlantic Area EAPA_791/2018_ NEUROATLANTIC projectINTER-REG V A España Portugal 0624_2IQBIONEURO_6_EXunta de Galicia IN856C2024/08, IN607A2022/07 and IN607D 2024/003
6 · The paper itself

Abstract

obejectiveTo characterize periodontal bacterial proteins in ischemic stroke thrombi and explore differences according to stroke etiology.

methodsThrombotic samples were obtained from nine severe periodontal patients with acute ischemic stroke (large-artery atherosclerosis, cardioembolic and undetermined source; 3 patients per stroke subtype) during mechanical thrombectomy. Samples were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Quantitative proteomic profiling was performed by sequential window acquisition of all theoretical mass spectra (SWATH-MS), focusing on proteins from five periodontal bacteria: Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Fusobacterium nucleatum, Tannerella forsythia, and Treponema denticola. Protein expression was compared across thrombus etiologies, and differentially expressed proteins were further analyzed in silico to explore their functions and interactions.

resultsA total of 136 bacterial proteins were quantified, predominantly from A. actinomycetemcomitans (112). Sixteen proteins showed differential expression (p < 0.05), mainly from this species. These were primarily associated with transcription, translation, and stress response mechanisms, including oxidative stress resistance. Few proteins from other species were differentially expressed, mostly related to structural and basic metabolic functions, except for a collagenase from T. forsythia in large-artery atherosclerosis stroke thrombi.

conclusionsSWATH-MS-based proteomic analysis revealed the presence of periodontal bacterial proteins in ischemic stroke thrombi, with a predominance of Aggregatibacter actinomycetemcomitans.

Indexed as

Bacterial ProteinsBrain IschemiaIschemic StrokePeriodontal DiseasesProteomicsThrombosisAgedAggregatibacter actinomycetemcomitansChromatography, LiquidFemaleFusobacterium nucleatumHumansMaleMiddle AgedPorphyromonas gingivalisTandem Mass SpectrometryBacterial ProteinsBacteriaCerebral ischemiaPeriodontitisProteomicsStrokeThrombosis

Identifiers

PMID42640357

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.