ArticleThe Eurasian journal of medicine2026
Real-World Concordance of Indirect Immunofluorescence and Antigen-Specific Assays in Antineutrophil Cytoplasmic Antibody Testing: A Retrospective High-Volume Laboratory Study.
Article in The Eurasian journal of medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCurrent guidelines recommend myeloperoxidase (MPO)- and proteinase 3 (PR3)-based immunoassays as the primary approach for antineutrophil cytoplasmic antibody (ANCA) testing, while indirect immunofluorescence (IIF) is reserved for selected clinical contexts. However, real-world data regarding concordance and workflow implications of these approaches remain limited.
methodsIn this retrospective laboratory-based study, ANCA results from 8976 consecutive patients evaluated in a high-volume tertiary laboratory were analyzed. Indirect immunofluorescence patterns and MPO/ PR3 reactivity were assessed simultaneously using an integrated IIF-based platform. Concordance between IIF patterns and antigen-specific reactivity was evaluated, and retrospective workflow simulations were performed to estimate the effects of MPO/PR3-first and IIF-first testing approaches on laboratory test utilization.
resultsOverall IIF positivity was observed in 589 patients (6.56%), whereas MPO and/or PR3 reactivity was detected in 307 patients (3.42%; 95% CI: 3.06-3.82%). Among IIF-positive patients, 438 (74.4%) showed no detectable MPO or PR3 reactivity, while 156 antigen-reactive patients (50.8%; 95% CI: 45.1-56.5%) showed no identifiable IIF reactivity. Retrospective workflow simulations suggested that an MPO/PR3-first approach would substantially reduce IIF testing volume, whereas an IIF-first strategy would markedly decrease antigenspecific testing volume. However, each approach was associated with distinct discordance patterns between IIF and MPO/PR3 reactivity within the study cohort.
conclusionSubstantial discordance was observed between IIF patterns and MPO/PR3 reactivity. The findings reflect laboratory concordance and workflow characteristics rather than true clinical diagnostic performance, and the clinical significance of discordant findings requires further evaluation in clinically characterized cohorts. Cite this article as: Arslan A, Togay A. Real-world concordance of indirect immunofluorescence and antigen-specific assays in antineutrophil cytoplasmic antibody testing: A retrospective high-volume laboratory study. Eurasian J Med. 2026, 58(4), 1498, doi: 10.5152/eurasianjmed.2026.261498.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.