ArticleVeterinary world2026
Comparative metagenomic and untargeted metabolomic analyses reveal gut microbiota and metabolite alterations associated with diarrhea in neonatal Holstein calves in Anhui, China.
Article in Veterinary world, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background and Aim: Neonatal calf diarrhea remains a major cause of morbidity, mortality, and economic losses in the dairy industry. Although alterations in gut microbial communities have been implicated in calf diarrhea, the interactions between intestinal microbiota and metabolites in neonatal Holstein calves remain incompletely understood. This study aimed to characterize gut microbiome and metabolomic alterations associated with diarrhea and to explore the relationships between differential microorganisms and metabolites in neonatal Holstein calves from Anhui, China. Materials and Methods: Fecal samples were collected from four diarrheic and four healthy female Holstein calves younger than 2 weeks of age. Shotgun metagenomic sequencing was performed using the DNBSEQ-T7 platform, and untargeted liquid chromatography-tandem mass spectrometry metabolomics was used to characterize fecal metabolites. Multivariate analyses, biomarker identification, pathway enrichment, and correlation analyses were conducted to investigate associations between microbial taxa and metabolites. Results: Distinct microbial and metabolic profiles were observed between healthy and diarrheic calves. Analysis of similarities confirmed significant differences in microbial composition between groups (R = 0.7917, p = 0.025). Forty microbial biomarkers were identified, with Conclusion: Neonatal calf diarrhea was associated with pronounced alterations in gut microbial communities and fecal metabolites. The enrichment of opportunistic pathogens and disruption of amino acid-related metabolic pathways highlight potential biomarkers and mechanistic links underlying gut dysbiosis. These findings provide novel insights into microbiota-metabolite interactions and may facilitate the development of targeted strategies to improve calf health and reduce economic losses in dairy production.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.