Estimation of DNA Methylation in Wheat Using Reverse Phase Ultra-High-Performance Liquid Chromatography Coupled with Mass Spectrometry During Leaf Rust Pathogenesis.

Uzma Afreen, Aryan Verma, Kunal Mukhopadhyay, Manish Kumar

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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5 · Who and what money

Authors and funding

4 authors.

Uzma AfreenDepartment of Bioengineering and Biotechnology, Birla Institute of Technology, Mesra, Ranchi, Jharkhand 835215, India.

Aryan VermaDepartment of Bioengineering and Biotechnology, Birla Institute of Technology, Mesra, Ranchi, Jharkhand 835215, India.

Kunal MukhopadhyayDepartment of Bioengineering and Biotechnology, Birla Institute of Technology, Mesra, Ranchi, Jharkhand 835215, India.

Manish KumarDepartment of Bioengineering and Biotechnology, Birla Institute of Technology, Mesra, Ranchi, Jharkhand 835215, India. manish@bitmesra.ac.in.ORCID http://orcid.org/0000-0002-3023-9018

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Reverse phase ultra-high-performance liquid chromatography is considered the most reliable and sensitive technique to determine and separate analytes with small differences in their molecular weights. Leveraging this technique coupled with mass spectrometry, we developed a method for simultaneous determination of 2'-deoxycytidine and its methylated analogue, 5-methyl-2'-deoxycytidine, in wheat. The nonpolar C

Indexed as

Chromatography, Reverse-PhaseDNA MethylationPlant DiseasesTriticumChromatography, High Pressure LiquidDeoxycytidineDNA, PlantLiquid Chromatography-Mass SpectrometryMass SpectrometryPlant LeavesPuccinia5-methyldeoxycytidineDeoxycytidineDNA, Plant2′-deoxycytidine5-methyl 2′-deoxycytidineCytosine MethylationLeaf rustUHPLC-MS/MSWheat

Identifiers

PMID42638016

DOI10.1007/s00425-016-2600-9

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.