ArticleMethods in molecular biology (Clifton, N.J.)2026
Analysis of Secreted Proteins from Macrophomina phaseolina Isolated from Infected Glycine max Tissues.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Necrotrophic fungi such as Macrophomina phaseolina (MP) pose a growing threat to global food security, especially as climate change expands their ecological range. MP is a highly adaptable phytopathogen that secretes a diverse array of metabolites and proteins-collectively known as the secretome-which play critical roles in host colonization and disease progression. In this chapter, we describe a method to evaluate the secretome of MP stimulated with ground Glycine max roots and seedlings, to identify proteins potentially implicated in the infection process. Current methods for analyzing proteins secreted by Mp do not incorporate supplementation with host plant tissue. However, including plant-derived substrates in secretion assays could stimulate the production of phytotoxic proteins that are otherwise not detected under standard conditions. These secreted effectors may play key roles in pathogenesis, and their function can be further validated through knockout mutagenesis or heterologous expression studies.
Indexed as
Identifiers
42638014What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.