ArticleMethods in molecular biology (Clifton, N.J.)2026
In Vitro Evaluation of Effectiveness of Antifungal Agents Against Macrophomina phaseolina.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antifungal agents provide both preventive and curative effects by protecting plant surfaces and eliminating existing infections within plant tissues. Evaluating their impact against phytopathogenic fungi is critical for sustainable agriculture. Macrophomina phaseolina is a highly destructive soil-borne pathogen with a broad host range, causing charcoal rot and related diseases that result in severe yield losses in crops such as soybean, sorghum, maize, and legumes. Several methods are employed to assess the in vitro antifungal efficacy of various substances, including broth microdilution, disk diffusion, gradient screening, and agar-based screening. However, these conventional techniques are generally slower, more labor-intensive, and limited by reproducibility issues. The adoption of microplate reader-based antifungal efficacy testing has enhanced efficiency and scalability, enabling high-throughput evaluation of multiple antifungal agents across a range of concentrations while providing a streamlined platform for preliminary screening. This chapter presents a convenient and cost-effective protocol for assessing the activity of antifungal agents against fungi that produce microsclerotia, with emphasis on standardization and quality control measures. The antifungal efficacy testing and cell death assay are employed to determine inhibitory concentrations via spectrophotometry and to quantify hyphal death through fluorescence microscopy, respectively. In addition, detailed procedures for inoculum preparation, antifungal agent dilution, incubation conditions, and statistical analysis are described.
Indexed as
Identifiers
42638011What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.