Evidence map›Paper›PMID 42638010›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Method for Analyzing CAZyme Activity from Whole-Body and Saliva Proteome of Russian Wheat Aphid.

Hlalanathi Y Gwanya, Lintle Mohase, Mpho S Mafa

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

3 authors.

Hlalanathi Y GwanyaCarbohydrates and Enzymology Laboratory (CHEM-LAB), Department of Plant Sciences, University of the Free State, P.O. Box 339, Bloemfontein, 9300, South Africa.
Lintle MohaseDepartment of Plant Science, University of the Free State, Faculty of Natural and Agricultural Sciences, Botany, Bloemfontein, South Africa.
Mpho S MafaCarbohydrates and Enzymology Laboratory (CHEM-LAB), Department of Plant Sciences, University of the Free State, P.O. Box 339, Bloemfontein, 9300, South Africa. mafams@ufs.ac.za.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Advanced proteome extraction and characterization techniques for Diuraphis noxia (Russian Wheat Aphid: RWA) enable precise biochemical profiling, where high-quality proteome extraction is critical. This chapter introduces an optimized proteome extraction method for comprehensive profiling of D. noxia, enabling analysis of whole-body and saliva proteomes to advance biochemical and functional characterization. The efficacy of two optimized proteome extraction methods was compared: a nondestructive artificial saliva extraction and a destructive whole-body extraction. The proteome extract concentration was quantified, and Carbohydrate-Active Enzymes (CAZymes) assays were performed to identify and quantify soluble sugar-degrading enzymes and cell wall-degrading enzymes. RWA is a phloem feeder, and this chapter demonstrates that it utilizes an enzyme cocktail consisting of invertase, trehalase, amyloglucosidase, and amylase during feeding. RWA is also equipped with cell wall-degrading enzymes in its proteome, such as cellulases and hemicellulases, which break down the cell wall, allowing the stylet to access the photoassimilates in phloem.

Indexed as

AphidsInsect ProteinsProteomeProteomicsSalivaTriticumAnimalsInsect ProteinsProteomeAphid saliva proteomeArtificial chamberDiuraphis noxiaRussian wheat AphidWhole-body aphid proteome

Identifiers

PMID42638010

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.