ArticleMethods in molecular biology (Clifton, N.J.)2026
Extraction of Amylases from Insects for Testing Inhibitory Compounds from Plants.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
α-Amylases (EC 3.2.1.1) are the hydrolytic enzymes that catalyze the breakdown of starch into maltose and glucose. In phytophagous insects, such as Helicoverpa armigera (cotton bollworm), α-amylases play a crucial role in carbohydrate digestion and, consequently, in larval growth and survival. Inhibition of these enzymes can impair nutrient assimilation, leading to reduced pest fitness. This chapter describes a clear step-by-step method to isolate α-amylases from H. armigera midguts, followed by the assessment of enzyme activity both quantitatively, using the classical Bernfeld dinitrosalicylic acid (DNS) assay, and qualitatively, using native-PAGE starch zymography. In-gel activity staining provides rapid visualization of distinct α-amylase isoforms and allows assessment of inhibitor effects directly on electrophoretic bands. This combined approach facilitates reproducible screening of α-amylases in diverse insect systems and provides a platform for evaluating plant-derived inhibitors for pest management applications.
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