Evidence map›Paper›PMID 42637730›Full record

ArticleNature communications2026

IDBac: an open-access web platform to identify bacteria and analyze relationships in culture collections using MALDI-TOF mass spectrometry.

Nyssa K Krull, Michael Strobel, Julia Saulog, Liana Zaroubi, Bruno S Paulo, Mandisa Timba, Douglas R Braun, Gabrielle Mingolelli, Jessia Raherisoanjato, Robert A Shepherd and 35 more

Abstract read
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

45 authors.

Nyssa K Krull *Department of Pharmaceutical Sciences, College of Pharmacy, University of Illinois Chicago, Chicago, IL, USA.
Michael Strobel *Department of Computer Science and Engineering, University of California Riverside, Riverside, CA, USA.
Julia SaulogDepartment of Chemistry, Simon Fraser University, Burnaby, BC, Canada.
Liana ZaroubiDepartment of Chemistry, Simon Fraser University, Burnaby, BC, Canada.
Bruno S PauloDepartment of Pharmaceutical Sciences, College of Pharmacy, University of Illinois Chicago, Chicago, IL, USA.ORCID http://orcid.org/0000-0002-1521-5034
Mandisa TimbaDepartment of Pharmaceutical Sciences, College of Pharmacy, University of Illinois Chicago, Chicago, IL, USA.
Douglas R BraunPharmaceutical Sciences Division, University of Wisconsin-Madison, Madison, WI, USA.
Gabrielle MingolelliDepartment of Pharmaceutical Sciences, College of Pharmacy, University of Illinois Chicago, Chicago, IL, USA.
Jessia RaherisoanjatoDepartment of Pharmaceutical Sciences, College of Pharmacy, University of Illinois Chicago, Chicago, IL, USA.
Robert A ShepherdDepartment of Chemistry & Biochemistry, University of California Santa Cruz, Santa Cruz, CA, USA.
Abigail F ScottDepartment of Pharmacology and Toxicology, University of Utah, Salt Lake City, UT, USA.ORCID http://orcid.org/0000-0001-9836-4184
Carlo De SilvaDepartment of Chemistry & Biochemistry, University of California Santa Cruz, Santa Cruz, CA, USA.ORCID http://orcid.org/0009-0009-3602-3259
Claire FergussonDepartment of Chemistry, Simon Fraser University, Burnaby, BC, Canada.
Zachary DanielCenter for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA, USA.
Shailaja K PokharelDepartment of Biomolecular Sciences, School of Pharmacy, University of Mississippi, Oxford, MS, USA.
Sean RomanowskiDepartment of Pharmaceutical Sciences, College of Pharmacy, University of Illinois Chicago, Chicago, IL, USA.
Antonio HernandezDepartment of Pharmaceutical Sciences, College of Pharmacy, University of Illinois Chicago, Chicago, IL, USA.ORCID http://orcid.org/0000-0002-7883-4852
Mónica Monge-LoríaSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Claire E DyllaDuchossois Family Institute, University of Chicago, Chicago, IL, USA.
Manasi M NatuDepartment of Chemistry & Biochemistry, University of California Santa Cruz, Santa Cruz, CA, USA.ORCID http://orcid.org/0009-0007-8303-7667
Valentina Z PetukhovaDepartment of Pharmaceutical Sciences, College of Pharmacy, University of Illinois Chicago, Chicago, IL, USA.
Chase M ClarkDepartment of Pharmaceutical Sciences, College of Pharmacy, University of Illinois Chicago, Chicago, IL, USA.
Neha GargSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.ORCID http://orcid.org/0000-0002-2760-7123
Paul R JensenCenter for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA, USA.ORCID http://orcid.org/0000-0003-2349-1888
Adriana BlachowiczInduced Environments Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA, USA.
Chelsi D CassillyNASA Marshall Space Flight Center, Huntsville, AL, USA.
Lisa GuanInduced Environments Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA, USA.
D Cole StevensDepartment of Biomolecular Sciences, School of Pharmacy, University of Mississippi, Oxford, MS, USA.ORCID http://orcid.org/0000-0001-6668-9419
Jaclyn M WinterDepartment of Pharmacology and Toxicology, University of Utah, Salt Lake City, UT, USA.ORCID http://orcid.org/0000-0001-6273-5377
Shaun M K McKinnieDepartment of Chemistry & Biochemistry, University of California Santa Cruz, Santa Cruz, CA, USA.ORCID http://orcid.org/0000-0001-6776-6455
Barbara I AdaikpohDepartment of Pharmaceutical Sciences, College of Pharmacy, University of Illinois Chicago, Chicago, IL, USA.
Skylar CarlsonDepartment of Chemistry, University of the Pacific, Stockton, CA, USA.
Erin P McCauleyDepartment of Chemistry and Biochemistry, California State University-Dominguez Hills, Carson, CA, USA.ORCID http://orcid.org/0000-0001-8474-7643
William W MetcalfDepartment of Microbiology, University of Illinois, Urbana, IL, USA.ORCID http://orcid.org/0000-0002-0182-0671
Tim S BugniPharmaceutical Sciences Division, University of Wisconsin-Madison, Madison, WI, USA.ORCID http://orcid.org/0000-0002-4502-3084
Michael W MullowneyDuchossois Family Institute, University of Chicago, Chicago, IL, USA.ORCID http://orcid.org/0000-0002-2884-4307
Eric G PamerDuchossois Family Institute, University of Chicago, Chicago, IL, USA.
Matthew T HenkeDepartment of Pharmaceutical Sciences, College of Pharmacy, University of Illinois Chicago, Chicago, IL, USA.
Hazel BartonDepartment of Geological Sciences, The University of Alabama, Tuscaloosa, AL, USA.ORCID http://orcid.org/0000-0001-8585-9997
David O CarterLaboratory of Forensic Taphonomy, Forensic Sciences Unit, School of Natural Sciences and Mathematics, Chaminade University of Honolulu, Honolulu, HI, USA.ORCID http://orcid.org/0000-0003-1885-5237
Alessandra S EustáquioDepartment of Pharmaceutical Sciences, College of Pharmacy, University of Illinois Chicago, Chicago, IL, USA.ORCID http://orcid.org/0000-0002-7852-7844
Roger G LiningtonDepartment of Chemistry, Simon Fraser University, Burnaby, BC, Canada.ORCID http://orcid.org/0000-0003-1818-4971
Laura M SanchezDepartment of Chemistry & Biochemistry, University of California Santa Cruz, Santa Cruz, CA, USA. lmsanche@ucsc.edu.ORCID http://orcid.org/0000-0001-9223-7977
Mingxun WangDepartment of Computer Science and Engineering, University of California Riverside, Riverside, CA, USA. mingxun.wang@ucr.edu.ORCID http://orcid.org/0000-0001-7647-6097
Brian T MurphyDepartment of Pharmaceutical Sciences, College of Pharmacy, University of Illinois Chicago, Chicago, IL, USA. btmurphy@uic.edu.ORCID http://orcid.org/0000-0002-1372-3887

Funding

Baccalaureate Bridge to the Biomedical Sciences Program (ACCESS)R25GM051765 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI CAMARA, JASON N., CREWS, PHIL · 1994 to 2023
$5.6M
MALDI-TOF MS/IDBac to discover antibiotics from the Great Lakes sponge microbiomeR01GM125943 · NIGMS · UNIVERSITY OF ILLINOIS AT CHICAGO · PI MURPHY, BRIAN THACHER, SANCHEZ, LAURA MARGARET · 2018 to 2021
$2.1M
Identification, characterization, and application of bacterial site-specific vanadium-dependent haloperoxidase enzymesR35GM147235 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI Shaun Mitchell Kirk McKinnie · 2022 to 2026
$1.9M
Development of a Bacterial Host for Natural Product Discovery and ProductionR01GM129344 · NIGMS · UNIVERSITY OF ILLINOIS AT CHICAGO · PI EUSTAQUIO, ALESSANDRA S · 2020 to 2023
$1.5M
Discovery of Novel Antibiotic Natural Products from Marine-Derived FungiSC2GM144172 · NIGMS · CALIFORNIA STATE UNIV-DOMINGUEZ HILLS · PI MCCAULEY, ERIN PATRICIA · 2022 to 2024
$441k
Development of a high throughput platform for screening directed evolution librariesR21GM148870 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI SANCHEZ, LAURA MARGARET · 2023 to 2024
$430k
NIGMS NIH HHS R01 GM125943NIGMS NIH HHS R01 GM129344NIGMS NIH HHS R21 GM148870NIGMS NIH HHS R25 GM051765NIGMS NIH HHS R35 GM147235NIGMS NIH HHS SC2 GM144172U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) R01GM125943U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) R01GM129344U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) R21GM148870U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) R25GM051765U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) R35GM147235U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) SC2GM144172
6 · The paper itself

Abstract

The identification and analysis of bacteria is central to the microbiological sciences. While gene sequencing methods have been the standard to achieve this, use of MALDI-TOF mass spectrometry (MS), particularly in clinical microbiology, can provide high-throughput identification to the subspecies level. However, biotyping has yet to be adopted outside of clinical settings due to the lack of a centralized public database of MS protein signatures that would facilitate isolate identification via spectral comparison. Further, most current MALDI MS data analysis platforms lack meaningful ways to compare properties from large numbers of bacterial isolates. Herein we present the IDBac web platform, a crowd-sourced central knowledgebase of protein MS signatures spanning seven bacterial phyla. Accompanying the knowledgebase is analysis infrastructure to identify unknown isolates, probe relationships within culture collections using metadata integration, and visualize specialized metabolite differences within groups of closely related bacteria. To highlight this utility and encourage wide community contribution, examples of each are presented.

Indexed as

BacteriaBacterial Typing TechniquesInternetSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationBacterial ProteinsSoftwareBacterial Proteins

Identifiers

PMID42637730
PMCPMC13503903

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.