Evidence map›Paper›PMID 42637222›Full record

ArticleAnimal science journal = Nihon chikusan Gakkaiho

Analysis of miRNA Expression in Bovine Endometrium During the Estrous Cycle.

Md Rafikul Islam, Manamu Takenouchi, Amir Salama, Takeshi Hayashi, Yoshinori Katakura, Kei Miyamoto, Nobuhiko Yamauchi

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In one paragraph

Article in Animal science journal = Nihon chikusan Gakkaiho. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Md Rafikul IslamDepartment of Animal and Marine Bioresource Sciences, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Manamu TakenouchiDepartment of Animal and Marine Bioresource Sciences, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Amir SalamaDepartment of Animal and Marine Bioresource Sciences, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Takeshi HayashiFukuoka Agriculture and Forestry Research Center, Fukuoka, Japan.
Yoshinori KatakuraDepartment of Bioscience and Biotechnology, Graduate School, Kyushu University, Fukuoka, Japan.ORCID https://orcid.org/0000-0002-9678-9602
Kei MiyamotoDepartment of Animal and Marine Bioresource Sciences, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.ORCID https://orcid.org/0000-0003-2912-6777
Nobuhiko YamauchiDepartment of Animal and Marine Bioresource Sciences, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.ORCID https://orcid.org/0000-0001-9254-2488

Funding

Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science 26292141
6 · The paper itself

Abstract

The present study aimed to identify and characterize bovine endometrial miRNAs at different stages of the estrous cycle. Total RNA was extracted from endometrial tissue and subjected to small RNA sequencing. A total of 334 miRNAs were expressed in bovine endometrial tissue during the estrous cycle. Among them, 11 were differentially expressed miRNAs (DEMs) at the follicular stage (F-DEMs) and four at the luteal stage (L-DEMs) in comparison between F and L stages. Bioinformatic analysis of the target genes of these DEMs revealed that miR-2411-3p and miR-320a were the top-ranked hub miRNAs in F- and L-DEMs, respectively. These two miRNAs demonstrated the highest functional coverage in GO annotation. The analysis using cultured bovine endometrial cells showed that the expression level of miR-2411-3p was significantly high in the E2-treated group compared to the control (p < 0.05), whereas the expression level of miR-320a was significantly high in the P4-treated group (p < 0.05). The present study identified the endometrial DEMs during the estrous cycle and predicted their functions based on their target genes. The results demonstrated that miR-2411-3p and miR-320a were identified as characteristic endometrial miRNAs regulated by ovarian steroid hormones in bovine.

Indexed as

EndometriumEstrous CycleGene ExpressionMicroRNAsAnimalsCattleCells, CulturedEstradiolFemaleFollicular PhaseLuteal PhaseProgesteroneEstradiolMicroRNAsProgesteronebovineE2, P4endometriumestrous cyclemiRNA

Identifiers

PMID42637222
PMCPMC13503040

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.