ArticleSynthetic biology (Oxford, England)2026
Integration-coupled activation of promoterless combinatorial pathway libraries in
Article in Synthetic biology (Oxford, England), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Combinatorial DNA design and assembly is an efficient and pragmatic way to obtain high-performing metabolic pathway designs quickly. However, implementation may require organism-specific technical barriers to be overcome. Firstly, suitable expression control parts such as promoters and ribosome-binding sites (RBSs), which provide a suitable range of expression levels, need to be identified or developed. Secondly, these need to be assembled into pathway-encoding combinatorial libraries of sufficient size, quality, and diversity. For organisms with transformation frequencies too low to allow direct transformation of library assembly reactions, such as many
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