ArticlemicroPublication biology2026
Cardiotoxic Effects of Acute 4.5 mM Caffeine Exposure in 2-to-3-Day-Old Zebrafish are Attenuated by Tricaine.
Article in microPublication biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
2 authors.
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Abstract
A previous study reported that 4 mM caffeine halted cardiac activity in 2-to-3-day-old zebrafish embryos by 72 minutes of exposure, although it remains unclear whether this resulted from a combined effect of caffeine and anesthesia. We demonstrate that 4.5 mM Caffeine significantly alters heart rate and rhythm in 2-to-3-day-old zebrafish embryos with or without tricaine anesthesia but rarely abolishes cardiac activity, even after ~24.5 hours of exposure. Notably, tricaine anesthesia co-administration significantly attenuated caffeine-induced bradycardia at ~2.5 hours and reduced the incidence of arrhythmias at both ~2.5 and ~24.5 hours post-treatment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.