Protocol for assessing molecular signatures and metabolic function of primary murine cortical astrocytes.

Nicole Blakeley, Baptiste Lacoste

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Nicole BlakeleyOttawa Hospital Research Institute, Regenerative Medicine & Neuroscience Programs, Ottawa, ON K1H 8L6, Canada; University of Ottawa, Department of Cellular and Molecular Medicine, Ottawa, ON K1H 8M5, Canada. Electronic address: nblak077@uottawa.ca.

Baptiste LacosteOttawa Hospital Research Institute, Regenerative Medicine & Neuroscience Programs, Ottawa, ON K1H 8L6, Canada; University of Ottawa, Department of Cellular and Molecular Medicine, Ottawa, ON K1H 8M5, Canada; University of Ottawa Brain Mind Research Institute, Ottawa, ON K1H 8M5, Canada. Electronic address: blacoste@uottawa.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Astrocytes are the most numerous brain cells and are central to the regulation of brain metabolism. Here, we present a protocol for identifying molecular signatures and assessing the metabolic function of primary murine cortical astrocytes. We describe steps for extracting, isolating and culturing astrocytes, characterizing these cells with immunocytochemistry, and extracting their metabolites. We then detail procedures for assessing mitochondrial function with challenges, RNA extraction for bulk sequencing, and DNA extraction for sequencing and epigenetics.

Indexed as

Cell-based AssaysCell BiologyCell cultureCell isolationMetabolomics

Identifiers

PMID42632024

DOI10.1016/j.xpro.2026.104793

PMCPMC13506315

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.