Evidence map›Paper›PMID 42632023›Full record

ArticleSTAR protocols2026

High-throughput protocol for assessing antibiotic resistance using agar dilution, acoustic liquid handling, and semi-automated image analysis.

Lieze Agten, Lieselotte Vermeersch, Philip Ruelens, Bram Van den Bergh, Natalie Verstraeten, Kevin Verstrepen, Jan Michiels

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Lieze AgtenKU Leuven, Department of Microbial and Molecular Systems (M2S), Centre of Microbial and Plant Genetics (CMPG), 3000 Leuven, Belgium; Symbiotic and Pathogenic Interaction Group, VIB Center for Microbiology, VIB, 3001 Leuven, Belgium. Electronic address: lieze.agten@kuleuven.be.
Lieselotte VermeerschKU Leuven, Department of Microbial and Molecular Systems (M2S), Centre of Microbial and Plant Genetics (CMPG), 3000 Leuven, Belgium; Lab for Systems Biology, VIB Center for Microbiology, VIB, 3001 Leuven, Belgium.
Philip RuelensKU Leuven, Department of Microbial and Molecular Systems (M2S), Centre of Microbial and Plant Genetics (CMPG), 3000 Leuven, Belgium; Symbiotic and Pathogenic Interaction Group, VIB Center for Microbiology, VIB, 3001 Leuven, Belgium.
Bram Van den BerghKU Leuven, Department of Microbial and Molecular Systems (M2S), Centre of Microbial and Plant Genetics (CMPG), 3000 Leuven, Belgium; Symbiotic and Pathogenic Interaction Group, VIB Center for Microbiology, VIB, 3001 Leuven, Belgium.
Natalie VerstraetenKU Leuven, Department of Microbial and Molecular Systems (M2S), Centre of Microbial and Plant Genetics (CMPG), 3000 Leuven, Belgium; Symbiotic and Pathogenic Interaction Group, VIB Center for Microbiology, VIB, 3001 Leuven, Belgium.
Kevin VerstrepenKU Leuven, Department of Microbial and Molecular Systems (M2S), Centre of Microbial and Plant Genetics (CMPG), 3000 Leuven, Belgium; Lab for Systems Biology, VIB Center for Microbiology, VIB, 3001 Leuven, Belgium.
Jan MichielsKU Leuven, Department of Microbial and Molecular Systems (M2S), Centre of Microbial and Plant Genetics (CMPG), 3000 Leuven, Belgium; Symbiotic and Pathogenic Interaction Group, VIB Center for Microbiology, VIB, 3001 Leuven, Belgium. Electronic address: jan.michiels@kuleuven.be.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Conventional minimum inhibitory concentration (MIC) assays used to determine the antibiotic susceptibility of microorganisms are inherently low-throughput and labor intensive. Here, we present a high-throughput agar dilution MIC assay to efficiently assess resistance profiles across large collections of bacterial isolates. We describe steps for agar plate preparation, culture spotting, imaging setup, and semi-automated image-based data analysis of this high-throughput assay. This approach enables simultaneous determination of antibiotic resistance levels for 384 strains across multiple antibiotics within a single workflow. For complete details on the use and execution of this protocol, please refer to Van den Bergh et al.

Indexed as

BioinformaticsCell cultureHealth sciencesHigh throughput screeningMicrobiologyMolecular biology

Identifiers

PMID42632023
PMCPMC13506316

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.