Evidence map›Paper›PMID 42632017›Full record

ArticleCell reports2026

16S ribosomal RNA modification drives transcript-specific translation efficiency.

Zachory M Park, Christina R Savage, Amanda R Decker-Farrell, Chin-Hsien Tai, Tapan K Maity, Weiming Yang, Lisa M Jenkins, Kumaran S Ramamurthi

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Article in Cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Zachory M ParkLaboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Christina R SavageLaboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Amanda R Decker-FarrellLaboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Chin-Hsien TaiLaboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Tapan K MaityLaboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Weiming YangLaboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Lisa M JenkinsLaboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Kumaran S RamamurthiLaboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA. Electronic address: ramamurthiks@mail.nih.gov.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bacterial ribosomal RNAs (rRNAs) are decorated with conserved nucleotide modifications, but the functionality of these modifications is often underexplored. MraW (RsmH) is a 16S rRNA methyltransferase. Here, we report that deletion of mraW corrects a late-stage sporulation defect in Bacillus subtilis by bypassing a sporulation checkpoint. Ribosomes purified from ΔmraW cells display a ∼2-fold decrease in translation efficiency; in vivo, ΔmraW cells produced decreased levels of the sporulation checkpoint protein CmpA. Reduced production of CmpA is mediated by mRNA sequences that form a stem-loop which occludes early cmpA codons. Proteomic analysis revealed that MraW mediates production of multiple proteins, some of whose mRNA form similar structures as the cmpA transcript. We propose that MraW modification of 16S rRNA enhances translation efficiency in general, and that specific transcripts have evolved structural features that fine-tune protein levels. This type of control may be prevalent in bacteria which exhibit uncoupled transcription and translation.

Indexed as

Bacillus subtilisProtein BiosynthesisRNA, Ribosomal, 16SBacterial ProteinsMethyltransferasesRibosomesRNA, BacterialRNA, MessengerSpores, BacterialBacterial ProteinsMethyltransferasesRNA, BacterialRNA, MessengerRNA, Ribosomal, 16S30S subunitCP: microbiologyCP: molecular biologyelongationinitiationRsmISpoIVASpoVMstationary phase

Identifiers

PMID42632017

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.