Evidence map›Paper›PMID 42631210›Full record

ArticleApplications in plant sciences

Expanded application to plant reproductive tissues of a branched DNA probe-based in situ hybridization method.

Brooklyn M Anaya, Austin T Nguyen, Kelly K S Matsunaga, Lena C Hileman

Abstract read
In one paragraph

Article in Applications in plant sciences. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Brooklyn M AnayaDepartment of Ecology and Evolutionary Biology University of Kansas Lawrence 66045 Kansas USA.ORCID https://orcid.org/0009-0007-5434-3403
Austin T NguyenDepartment of Ecology and Evolutionary Biology University of Kansas Lawrence 66045 Kansas USA.ORCID https://orcid.org/0009-0009-5644-726X
Kelly K S MatsunagaDepartment of Ecology and Evolutionary Biology University of Kansas Lawrence 66045 Kansas USA.ORCID https://orcid.org/0000-0002-9533-2934
Lena C HilemanDepartment of Ecology and Evolutionary Biology University of Kansas Lawrence 66045 Kansas USA.ORCID https://orcid.org/0000-0001-6116-666X

Funding

Mentoring CoreP20GM103418 · NIGMS · UNIVERSITY OF KANSAS MEDICAL CENTER · PI SUSAN J. BROWN · 2012 to 2026
$63.0M
Synthetic Chemical Biology CoreP30GM145499 · NIGMS · UNIVERSITY OF KANSAS LAWRENCE · PI Susan M Lunte · 2022 to 2026
$6.9M
NIGMS NIH HHS P20 GM103418NIGMS NIH HHS P30 GM145499
6 · The paper itself

Abstract

Premise: Detecting clear tissue- and organ-specific patterns of gene expression is key to understanding the genetic mechanisms that control plant development. In situ hybridization (ISH) of mRNA is one of the most precise, yet most challenging approaches to gene expression assays. Methods and Results: Detection of histone H4 expression in reproductive tissues of Conclusions: The RNAscope assay can quickly and sensitively generate high-quality ISH results in reproductive tissues across a breadth of plant species.

Indexed as

branched DNA probesCYCLOIDEAhistone H4inflorescencein situ hybridizationreproductive coneRNAscopeZ‐probe

Identifiers

PMID42631210
PMCPMC13495965

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.